Objective:To establish an HPLC method for simultaneous determination of gentiopicroside

loganic acid

swertiamarin and sweroside in Radix Gentianae. Method: Chromatographic separation was achieved on an Inertsil ODS-SP column (4.6 mm×150 mm

5 μm) with methanol (A)-0.4% phosphoric acid (B) as mobile phase for gradient elution (0-40 min

10%-30%A) at a flow rate of 1.0 mL·min-1. The column temperature was maintained at 25 ℃ and the detecting wavelength was set at 240 nm. Result: The linear ranges were 1.0-20 μg (r=0.999 7) for gentiopicroside

1.0-20 μg (r=0.999 6) for loganic acid

1.0-20 μg (r=0.999 5) for swertiamarin

0.1-2.0 μg (r=0.999 5) for sweroside. The average recoveries of four components were 99.1%

99.0%

98.9% and 98.5%

respectively. Conclusion: The developed method is accurate

sensitive

credible

and repeatable. It can be applied to the quality control of Radix Gentianae.