Determination of ZP-7 Tenacigenin B in Marsdeniae Tenocissimae by HPLC

ZHANG Hui; PEI Zhi-dong; WANG Tian-min; QIAO Jia

doi:10.11653/syfj2013130088

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Determination of ZP-7 Tenacigenin B in Marsdeniae Tenocissimae by HPLC

更新时间：2024-01-04

- Determination of ZP-7 Tenacigenin B in Marsdeniae Tenocissimae by HPLC
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 19, Issue 13, Pages: 88-90(2013)
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- DOI：10.11653/syfj2013130088
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- Published：2013
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ZHANG Hui, PEI Zhi-dong, WANG Tian-min, et al. Determination of ZP-7 Tenacigenin B in Marsdeniae Tenocissimae by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(13): 88-90.
DOI：

ZHANG Hui, PEI Zhi-dong, WANG Tian-min, et al. Determination of ZP-7 Tenacigenin B in Marsdeniae Tenocissimae by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(13): 88-90. DOI： 10.11653/syfj2013130088.

摘要

目的:建立HPLC测定通关藤药材中ZP-7通关藤苷元B([11α-O-(2-甲基丁酰基)-12β-O-乙酰基通关藤苷元B])含量的方法

并对不同产地的药材进行测定。方法:采用Agilent TC-C18柱 (4.6 mm×150 mm

5 μm)色谱柱

流动相乙腈-水(35∶65)

流速1.0 mL·min-1

检测波长210 nm

柱温25 ℃

进样量10 μL。结果: ZP-7通关藤苷元B的线性范围为3.183~7.427 μg(r=0.999 4)

平均回收率 99.95%(RSD 0.83%)。结论:方法准确

重复性好

专属性强

可控制药材的质量。

Abstract

Objective:To establish a HPLC method for the content determination of ZP-7 tenacigenin B in Marsdeniae Tenocissimae

and to determine the content of them in Marsdeniae Tenocissimae from different areas. Method: The analytical mothod was carried out with Agilent TC-C18(4.6 mm×150 mm

5 μm) column and the mobile phase consisted of acetonitrile-water(35∶65)at a flow rate of 1.0 mL·min-1. The detection wavelength was set at 210 nm

the column temperature was 25 ℃ and injection volume was 10 μL. Result: The calibration curves was linear within the range of 3.183-7.427 μg (r=0.999 4) for ZP-7 tenacigenin B. The average recovery was 99.95% (RSD 0.83%). Conclusion: The method is accurate

reproducible and precise

and can control the content of ZP-7 tenacigenin B in Marsdeniae Tenocissimae

and can guide the clinical medication.

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Related Institution

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