Objective:To establish an HPLC fingerprint of Euonymus alatus analyzed the chemical constituents what these peaks stood for and compared HPLC fingerprint of E. alatus in different parts. Method: The determination was carried out with Diamonsil C18 (4.6 mm×250 mm

5 μm) column

using acetonitrile-0.1% acetic acid as mobile phase with gradient elution at a flow rate of 1.0 mL·min-1 and detected at the wavelength of 254 nm and temperature was maintained at 30 ℃. The chromatograms were analyzed with the software of Estimating System of Similarity of 2004A Version (the Country's Pharmacopeia Committee) on the Chinese Medicine Fingerprint Chromatography. Result: The HPLC fingerprint was established by evaluating the chromatogram of 9 samples from different regions and there were 23 common peaks showed in the fingerprint chromatogram. The similarity of 9 batches of E. alatus were 0.824-0.957 in which 6 sources were above 0.900. Conclusion: The constituents in the different parts of E. alatus were so different comparing their HPLC fingerprint that the quality control of E. alatus should be established on the basis of its clinical applications.