WANG Kun-feng, LIN Chen, WU Ling-fang, et al. Determination of Total Phenols from in Different Locations[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(13): 110-112.
DOI:
WANG Kun-feng, LIN Chen, WU Ling-fang, et al. Determination of Total Phenols from in Different Locations[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(13): 110-112. DOI: 10.11653/syfj2013130110.
Determination of Total Phenols from in Different Locations
Objective:To establish a method for determination of total phenols from Phyllanthus urinaria in different locations. Method:Total phenols was determined by UV-Vis spectrophotometer with corilagin as reference substance and FeCl3-K3[Fe(CN)6] as chromogenic agent
the detection wavelength was at 744 nm. Result:A good linear relationship of corilagin was achieved in the range of 0.604-3.02 mg·L-1 (r=0.999 1)
the average recovery was 99.79% with RSD of 2.72%(n=6).The content of total phenols from P. urinaria in different locations was different from 5.00% to 12.16%. Conclusion:This established method was simple
accurate and stable which was suitable for determination the content of total phenols from P. urinaria.
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