Objective:To establish a method for simultaneous determining of berberine hydrochloride

phellodendrine hydrochloride and matrine in Qingfei Yihuo pills by capillary electrophoresis. Method: With pseudoephedrine hydrochloride as the internal standard

separation was performed on a uncoated fused silica capillary of 55 cm×75 μm(47 cm effective length). 100 mmol·L-1 NaH2PO4+40% ethanol solution(pH 5.75) was selected as the running buffer

separation voltage was 23 kV

samples was injected by gravity in 15 s (15 cm)

detection wavelength was at 208 nm. Result: Calibration curves for berberine hydrochloride

phellodendrine hydrochloride and matrine were linear in the concentration range of 40.5-81.0

0.896-3.584

6.0-36.0 mg·L-1.The average recoveries were 96.0%

100.5%

97.8%

respectively

with RSD were not more than 1.85%. Conclusion: This method was accurate and reliable with good repeatability

it could be used for quantitative determination of Qingfei Yihuo pills.