Simultaneous Determination of Baicalin and Icariin in Fuzheng Guben Granule by HPLC

LI Jian-wei; HAI Li-na; NIE Lei; LIU Juan

doi:10.11653/syfj2013130128

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Simultaneous Determination of Baicalin and Icariin in Fuzheng Guben Granule by HPLC

更新时间：2024-01-04

- Simultaneous Determination of Baicalin and Icariin in Fuzheng Guben Granule by HPLC
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 19, Issue 13, Pages: 128-130(2013)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.11653/syfj2013130128
  CLC：
- Published：2013
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LI Jian-wei, HAI Li-na, NIE Lei, et al. Simultaneous Determination of Baicalin and Icariin in Fuzheng Guben Granule by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(13): 128-130.
DOI：

LI Jian-wei, HAI Li-na, NIE Lei, et al. Simultaneous Determination of Baicalin and Icariin in Fuzheng Guben Granule by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(13): 128-130. DOI： 10.11653/syfj2013130128.

摘要

目的: 建立扶正固本颗粒中黄芩苷和淫羊藿苷的含量测定方法。方法: 采用HPLC法

以乙腈-0.2%磷酸溶液梯度洗脱

色谱柱为日旭AQ C18(4.6 mm×250 mm

5 μm)

流速1.0 mL·min-1

柱温25 ℃

检测波长270 nm。结果: 黄芩苷在0.756~10.08 μg

淫羊藿苷在0.299~39.92 μg与峰面积呈良好线性关系

Abstract

Objective:To develop a quantitative method for determining the contents of baicalin and icariin in Fuzheng Guben granule. Method: The analysis was performed with a Ultimate AQC18(4.6 mm×250 mm

5 μm) column and a mobile phase of acetonitrile-0.2% phosphoric acid solution with gradient elution. The flow rate was 1.0 mL·min-1

and column temperature was 25 ℃. The detection wavelength was set at 270 nm. Result: The calibration curve was linear within the range of 0.756-10.08 μg for baicalin(r=1.000 0) and 0.299-39.92 μg for icariin(r=0.999 6). The average recoveries of the two contents were 101.40%(RSD 1.41%)and 101.02%(RSD 1.08%) respectively. Conclusion: This method is convenient

accurate and reliable

and can be used as quality control methods for Fuzheng Guben granule.

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Related Author

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LUO Yu-jian

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Related Institution

Department of Pharmacy,First Affiliated Hospital of PLA General Hospital

Beijing Institute For drug Control

School of Traditional Chinese Medicine,Capital Medical University

Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences

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