Simultaneous Determination of Three Chemical Constitutes in Qingfei Yihuo Pills by HPLC

CHEN Jun; XU Hua; CHEN Xiao-xuan

doi:10.11653/syfj2013130134

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Simultaneous Determination of Three Chemical Constitutes in Qingfei Yihuo Pills by HPLC

更新时间：2024-01-04

- Simultaneous Determination of Three Chemical Constitutes in Qingfei Yihuo Pills by HPLC
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 19, Issue 13, Pages: 134-136(2013)
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- DOI：10.11653/syfj2013130134
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- Published：2013
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CHEN Jun, XU Hua, CHEN Xiao-xuan. Simultaneous Determination of Three Chemical Constitutes in Qingfei Yihuo Pills by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(13): 134-136.
DOI：

CHEN Jun, XU Hua, CHEN Xiao-xuan. Simultaneous Determination of Three Chemical Constitutes in Qingfei Yihuo Pills by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(13): 134-136. DOI： 10.11653/syfj2013130134.

摘要

目的: 建立清肺抑火丸中3个苷类成分的含量标准。方法: 采用HPLC

同时以2个检测波长对清肺抑火丸中的黄芩苷、京尼平苷、京尼平龙胆二糖苷进行定量分析。Agilent Zorbax SB-C18色谱柱(4.6 mm×250 mm

5 μm);流动相甲醇(A)-1%甲酸溶液(B)-乙腈(C)梯度洗脱

检测波长238

278 nm。结果: 黄芩苷、京尼平苷、京尼平龙胆二糖苷分别在0.165~1.650

0.106~1.060

0.035~0.350 μg(r>0.999 7)线性关系良好

平均回收率>96.75%

RSD<2.05%。结论: 该方法同时进行黄芩苷、京尼平苷、京尼平龙胆二糖苷的定量分析

可作为清肺抑火丸的质量控制标准。

Abstract

Objective:To establish a HPLC method for determination of three constituents in Qingfei Yihuo Pills. Method: Baicalin

geniposide and genipin-1-O-β-gentiobioside were determined simultaneously by HPLC on a agilent Zorbax SB-C18 column with the methanol(A)-1.0% formic acid anhydrous(B)-acetonitrile(C) in gradient elution as the mobile phrase. The detection wavelength was set at 238

440 nm and the flow rate was 1.0 mL·min-1. Result: The standard curves for baicalin

geniposide and genipin-1-O-β-gentiobioside were linear within the range of 0.165-1.650

0.106-1.060

0.035-0.350 μg (r>0.999 7)

and the average recoveries were over 96.75% (RSD<2.05%). Conclusion: The method is simple and accurate and could be used for quality control of Qingfei Yihuo Pills.

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Related Author

TANG De-zhi

CAO Yang-yang

YAN Ren-yi

YANG-Bin

HUANG Lu-qi

CAO Yang-yang1

YAN Ren-yi2

YANG-Bin2

Related Institution

School of Traditional Chinese Medicine,Capital Medical University

Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences

Nanning institute for Food and Drug Control of Guangxi

Research Center for Differentiation and Development of Traditional Chinese Medicine （TCM） Basic Theory， Jiangxi Province Key Laboratory of TCM Etiopathogenisis， Jiangxi University of Chinese Medicine

Henan Engineering Research Center for Quality Control and Evaluation of Traditional Chinese Medicine（TCM），School of Pharmacy，Henan University of Chinese Medicine

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