Objective:To establish a HPLC fingerprint method of Sargentodoxa cuneata

for its quality control. Method: The separation was performed by RE-HPLC chromatography Ultimate XB-C18 (4.6 mm× 250 mm

5 μm) column with a mobile phase of acetonitrile-methanol (1∶1) and 0.1% phosphoric acid water at a flow rate of 0.6 mL·min-1 and 25 ℃

gradient elution. The detecting wave length was 280 nm. The Milettia reticulata and Longpeduncle kadsura stem fingerprints were also detected for similarity comparison. application system cluster analysis to classify different sources S. cuneata herbs. Result: The HPLC fingerprint analysis method of S. cuneata was established and Calibration of 22 common peaks

identified three peaks:salidroside

protocatechuic and Rhein. There were significant differences in fingerprint chromatogram of S. cuneata

M. reticulata and L. kadsura stem.11 batches of different sources S. cuneata herbs can be divided into three categories by Hierarchical cluster analysis. Conclusion: The method is simple

accurate

reproducible

and can be used to the S. cuneata fingerprint research.