Membrane Transport of the Icariin, which is Main Component of Naoyiyuan Tables Across the Caco-2 Monolayer

ZHOU Gai-lian; SHAN Gui-zhi; LIN Li; WANG Lian-jing

doi:10.11653/syfj2013130178

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Membrane Transport of the Icariin, which is Main Component of Naoyiyuan Tables Across the Caco-2 Monolayer

更新时间：2024-01-04

- Membrane Transport of the Icariin, which is Main Component of Naoyiyuan Tables Across the Caco-2 Monolayer
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 19, Issue 13, Pages: 178-181(2013)
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- DOI：10.11653/syfj2013130178
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- Published：2013
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ZHOU Gai-lian, SHAN Gui-zhi, LIN Li, et al. Membrane Transport of the Icariin, which is Main Component of Naoyiyuan Tables Across the Caco-2 Monolayer[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(13): 178-181.
DOI：

ZHOU Gai-lian, SHAN Gui-zhi, LIN Li, et al. Membrane Transport of the Icariin, which is Main Component of Naoyiyuan Tables Across the Caco-2 Monolayer[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(13): 178-181. DOI： 10.11653/syfj2013130178.

摘要

目的: 对复方脑忆源片中主要成分淫羊藿苷进行转运研究

阐明吸收特点。方法: 利用Caco-2细胞模型

对复方脑忆源片中主要成分淫羊藿苷(ICA)进行体外转运

并通过高效液相(Luna Kromasil C18色谱柱(4.6 mm×150 mm

5 μm);流动相乙腈∶水(30∶70);流速1.0 mL·min-1;检测波长270 nm;)测定单体、药材及复方中的淫羊藿苷转运后的含量。结果: 淫羊藿苷在小肠吸收转运过程随时间增长而增加

可能会被Caco-2细胞中的P-gp转运蛋白外排。与淫羊藿单体和淫羊藿提取物中的淫羊藿苷比较

脑忆源片可显著提高淫羊藿苷的转运

同时可降低淫羊藿苷的外排作用。结论: 在脑忆源片中

由于淫羊藿苷外排减少

可认为通过复方配伍后

方中的其他药物抑制了淫羊藿苷的外排

使其可以更好的发挥药理作用。

Abstract

Objective:The absorption characteristic of the icariin(ICA)

which is the main component of Naoyiyuan tables

was clarified by the membrane transport study. Method: The Caco-2 monolayer was used to study the transport of the ICA in monomer

herbs

and compounds in vitro.For the determination of the content of ICA

the HPLC method was operated with acetonitrile-water(30∶70) as mobile phase

with Luna Kromasil C18 column. The flow rate was 1.0 mL·min-1

detection wavelength was at 270 nm. Result: ICA exhibited time-dependent permeation with apparent permeability coefficient P-gp values in the range 1×10-5-1×10-6cm·s-1

suggesting transcellular diffusion pathways. P-glycoprotein may play a role in the intestinal efflux of icariin. However compared with the icariin in monomer and herbs

the transport of ICA in Naoyiyuan tables is higher. Conclusion: The intestinal efflux of ICA in Naoyiyuan tables was reduced. So we can considered that the other ingredients in Naoyiyuan tables inhibited the efflux of icariin. It is profit for the pharmacological actions.

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