Objective:To compare the differences between the asthenia-cold syndrome in patients with primary dysmenorrhea and normal group in urinary endogenous metabolites

and elaborate the path physiological mechanisms of the asthenia-cold syndrome in patients with primary dysmenorrheal. Method: N-methyl-N-(trimethylsilyl) tri-fluoroacetamide (MSTFA) was selected as derivatization reagent to detect urine metabolites at the second day in menstrual cycle (MC2) of the two groups using gas chromatography-time of flight mass spectrometer (GC-TOFMS)

drawn the total ion chromatogram(TIC) of each group

combination with unsupervised principal component analysis (PCA) for the multi-dimensional statistical analysis to look for variability material between the two groups. Result: Compared with the normal group

twelve variability materials in asthenia-cold group were detected. They were fructose

maltose

arbinofuranose

isocitric acid

lactic acid

hippuric acid

glycine

serine

threonine

palmitic acid

octadecanoic acid

and erythritol. Among the total materials only lactic acid in asthenia-cold group was higher than in the normal group;the rest materials in asthenia-cold group were all lower than in the normal group. Conclusion: Asthenia-cold syndrome of the primary dysmenorrheal patients and the normal group suggested significant difference in urine metabolites. These variability materials are related to several metabolic pathways such as glycolmetabolism

amino acid metabolism

fatty acid metabolism and disturbance of enterobacteria.