Expressions of Core Binding Factor Alpha 1 mRNA of Femoral Organization by Zuogui Pill in Ovariectomy-induced Osteoporosis Rats

WANG Yan-jie; REN Yan-ling; SONG Nan; LV Hai-bo; LI Ya-ling

doi:10.11653/syfj2013130191

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Expressions of Core Binding Factor Alpha 1 mRNA of Femoral Organization by Zuogui Pill in Ovariectomy-induced Osteoporosis Rats

更新时间：2024-01-04

- Expressions of Core Binding Factor Alpha 1 mRNA of Femoral Organization by Zuogui Pill in Ovariectomy-induced Osteoporosis Rats
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 19, Issue 13, Pages: 191-195(2013)
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- DOI：10.11653/syfj2013130191
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- Published：2013
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WANG Yan-jie, REN Yan-ling, SONG Nan, et al. Expressions of Core Binding Factor Alpha 1 mRNA of Femoral Organization by Zuogui Pill in Ovariectomy-induced Osteoporosis Rats[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(13): 191-195.
DOI：

WANG Yan-jie, REN Yan-ling, SONG Nan, et al. Expressions of Core Binding Factor Alpha 1 mRNA of Femoral Organization by Zuogui Pill in Ovariectomy-induced Osteoporosis Rats[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(13): 191-195. DOI： 10.11653/syfj2013130191.

摘要

目的: 观察左归丸对去卵巢骨质疏松大鼠股骨中核心结合因子α1 mRNA表达的影响

探讨其对骨质疏松的防治机制。方法: 280只SPF级SD雌性大鼠

分为3组:正常组 40只、假手术组40只、其余采取双侧背部卵巢切除术进行绝经后骨质疏松症造模。21 d后

随机分为5组:模型组、尼尔雌醇组、左归丸高、中、低剂量组。左归丸高、中、低剂量组分别给予ig 6.4

3.2

1.6 g·kg-1剂量的左归丸混悬液

1次/d;尼尔雌醇组ｉｇ尼尔雌醇混悬液0.21 mg·kg-1

每周1次。于连续给药60

120

180 d

分别取大鼠左后肢股骨近端1/3部分

采用RT-PCR 方法测定核心结合因子α1的mRNA表达水平。结果: 与正常组比较

模型组股骨中核心结合因子α1 mRNA水平明显降低(P＜0.01);和模型组比较

给药60

120

180 d的左归丸各剂量组

股骨中核心结合因子α1 mRNA 水平显著升高(P＜0.05)。结论: 骨组织中核心结合因子α1 mRNA表达水平的下调可能是PMOP发生的重要机制之一

左归丸能够上调骨组织中核心结合因子α1 mRNA表达

从而有效防治骨质疏松。

Abstract

Objective:To observe the expression of core binding factor alpha 1 mRNA of femoral organization by Zuogui Pill in ovariectomy-induced osteoporosis rats. Method: Two hundred and eighty male SPF SD rats were divided into three groups:normal group 40;sham-operated group 40;ovariectomy-induced osteoporosis model was adopted

21 days later

randomly modeling rats were divided into 5 groups:the model group

nilestriol group

Zuogui Pill high

medium

low dose group. Zuogui Pill groups were ig given with Zuogui Pill at dosage of 6.4

3.2

1.6 g·kg-1

once per day. Nilestriol group was treated with Nilestriol liquid. The normal group

sham-operated group and the model group were given with distilled water.60

120

180 d later

the expression of core binding factor alpha 1 in the femoral organization was determined by RT-polymerase chain reaction (PCR). Result: Compared with the normal group

in the model group the femoral core binding factor alpha 1 mRNA level obviously reduced (P<0.01). And compared with the model group

Zuogui Pill groups increased femoral core binding factor alpha 1 mRNA level significantly(P<0.05). Conclusion: The down-regulated expression of core binding factor alpha 1 mRNA of bone tissue may be contributed to PMOP

Zuigui Pill can up-regulated mRNA expression.

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