Effects of Yulangsan Polysaccharides on Caspase-3 Expression and Activity in the Frontal Lobe and Hippocampus of SAMP8 Mice

CHEN Xiao-yu; HUANG Ren-bin

doi:10.11653/syfj2013130208

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Effects of Yulangsan Polysaccharides on Caspase-3 Expression and Activity in the Frontal Lobe and Hippocampus of SAMP8 Mice

更新时间：2024-01-04

- Effects of Yulangsan Polysaccharides on Caspase-3 Expression and Activity in the Frontal Lobe and Hippocampus of SAMP8 Mice
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 19, Issue 13, Pages: 208-211(2013)
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- DOI：10.11653/syfj2013130208
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- Published：2013
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CHEN Xiao-yu, HUANG Ren-bin. Effects of Yulangsan Polysaccharides on Caspase-3 Expression and Activity in the Frontal Lobe and Hippocampus of SAMP8 Mice[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(13): 208-211.
DOI：

CHEN Xiao-yu, HUANG Ren-bin. Effects of Yulangsan Polysaccharides on Caspase-3 Expression and Activity in the Frontal Lobe and Hippocampus of SAMP8 Mice[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(13): 208-211. DOI： 10.11653/syfj2013130208.

摘要

目的: 考察玉郎伞多糖(YLSP)对快速老化(SAMP8)小鼠额叶、海马神经细胞半胱天冬蛋白酶-3(Caspase-3)表达及活性的影响。方法: 选择6月龄SAMP8小鼠

随机分为模型组

石杉碱甲组(0.02 mg·kg-1)

YLSP低、高剂量组(45

180 mg·kg-1)

选择6月龄正常老化小鼠(SAMR1)作为对照组

以上各组每日上午ig给药l次

连续40 d

于末次给药的次日应用免疫组织化学方法检测额叶和海马Caspase-3的表达变化及神经细胞凋亡情况

测定Caspase-3活性。结果: SAMP8小鼠额叶和海马Caspase-3阳性细胞数分别为(46.00±5.30)

(51.36±3.28)个

Caspase-3活性分别为2 380±193

2 473±241

均较对照组显著升高(4.90±0.60)

(6.14±0.53);(437±76)

(521±79)(P<0.01);与模型组相比

石杉碱甲组、YLSP低、高剂量组额叶和海马Caspase-3阳性细胞数(23.48±3.46

27.86±4.04;24.54±3.55

30.60±2.62;17.98±2.97

23.90±2.58)及Caspase-3活性(1323±126

1 456±130;1 397±133

1 483±147;987±79

1 091±95)明显低于模型组(P<0.01);与石杉碱甲组、YLSP低剂量组比较

YLSP高剂量组的额叶和海马Caspase-3阳性细胞数及Caspase-3活性显著降低(P<0.05)。结论: 表明YLSP降低Caspase-3表达及活性

减少SAMP8小鼠脑部神经细胞凋亡。

Abstract

Objective:The effects of Yulangsan polysaccharides(YLSP) on Caspase-3 expression and activity of the frontal lobe and hippocampus in senescence accelerated-prone mouse 8(SAMP8) mice brain were investigated. Method: The SAMP8 mice were adopted as the Alzheimer's disease model group

and SAMR1 as the control group. Both groups were randomized and given 0.5%CMC-Na solution. Huperzine(0.02 mg·kg-1) was given to drug-positive group and two YLSP groups were given 45

180 mg·kg-1 respectively

continuous administration for 40 days. In the frontal lobe and hippocampus

Caspase-3 expression and activity were detected respectively by immunohistochemical method and Caspase-3 activity assay kit. Result: In model group

Caspase-3 expression and activity were obviously higher than that in the control group(P<0.01). In drug-positive and YLSP groups

Caspase-3 expression and activity were obviously lower than that in model group(P<0.01). YLSP presented a dose-dependent manner (P<0.05). Conclusion: The abnormal Caspase-3 expression and activity can be reduced by YLSP

and it has the protective function towards neural cell apoptosis in SAMP8 mouse brain.

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