Objective:To study the anti-cancer activity of two flavonoids 4'-methoxy-eupatolitin-3-O-β-D-glucoside(compoundⅠ)and kaempfrol-3

7-O-α-L-di-rhamnoside(compoundⅡ) in the leaf and stem of Tagetes erecta. Method: SGC7901 and SMMC7721 cells were treated by 20

40

80

120

160 μmol·L-1 two flavonoids for 24

48

72 h

MTT assay was used to determine the inhibition rates

and 5-FU was used as positive control. AO/EB double fluorescent staining was used to observe the changes of cell morphology and the apoptosis of tumor cells at the concentration of 1×106/mL after treated with 160 μmol·L-1 of two flavonoids for 48 h. Result: The different concentrations of two flavonoids could both inhibit the proliferation of SGC7901 and SMMC7721 in the time-and dose-dependent manner.The IC50 of compoundⅠ was 111.7 mg·L-1 and 330.4 mg·L-1

respectively. The IC50 of compoundⅡwas 683.8 mg·L-1 and 464.7 mg·L-1

respectively.The morphology of two tumor cancer cells was changed after treated by two compounds

and part of them emerged apoptosis. Conclusion: Two flavonoids separated from the leaf and stem of T. erecta have the activity of anti-liver cancer and anti-gastric cancer in vitro.