WU Ruo-xia, WANG Yun-xin, CHEN Hui, et al. Expression of bFGF in Myocardial Cells by Containing Jiawei Danshen Yin Serum in BMSCs Transplanted IRI Rats[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(13): 238-242.
DOI:
WU Ruo-xia, WANG Yun-xin, CHEN Hui, et al. Expression of bFGF in Myocardial Cells by Containing Jiawei Danshen Yin Serum in BMSCs Transplanted IRI Rats[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(13): 238-242. DOI: 10.11653/syfj2013130238.
Expression of bFGF in Myocardial Cells by Containing Jiawei Danshen Yin Serum in BMSCs Transplanted IRI Rats
Objective:To observe effect of Jiawei Danshen Yin(JWDSY) on the expression of basic fibroblast growth factor (bFGF) in the edge of infarcted myocardium in the rats with ischemia reperfusion injury(IRI) which was transplanted in bone mesenchymal stem cells(BMSCs). Method: BMSCs were cultivated by bone marrow adherent method. The phenotypes of CD90
CD11b
and CD45 were analyzed with flow cytometer. The third generation cells were treated with blank serum or containing JWDSY serum. Rats were modeled as IRI
and then BMSCs were transplanted. In 3 d
7 d
14 d
respectively
specimens were taken from each group
myocardial bFGF were detected. Result: IA value of containing JWDSY serum group after 3
7
14 day increased to 5 758.18±465.17
6 589.61±432.89
8 015.62±366.34 respectively. Compared with sham operation groups
the IA value of the rest groups were raised as time gradually
the difference had statistics meanings (P<0.05). Conclusion: The results suggested that BMSCs intervened by containing JWDSY serum could protect the myocardial cells in the IRI rats.
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