Objective:Study the way to make Huizhuo Tanzu type atherosclerosis animal model. Method: Divide 60 ApoE gene knock out mouse into 4 groups: high fat diet and cold dry environment intervention animal model group

high fat diet control group

cold dry environment intervention control group and normal control group. 12 weeks after nurturing in different groups

the weight

emotion

defecation

tongue presentations were observed in all animals.Biochemical indicators and cell morphology were compared between groups after sacrificing all the animals. Result: Indicators of Huizhuo Tanzu type atherosclerosis in High fat diet and cold dry environment intervention animal model group animals are significantly higher than other groups (P<0.05) . Light microscopic observations revealed that all the animals developed atheromatous plaque. high fat diet and cold dry environment intervention alone has significantly different affect on the development of atheromatous plaque comparing to a combined intervention of both high fat diet and cold dry environment (ANOVA

P<0.05). Conclusion: Animal underwent combined intervention of high fat diet and cold dry environment significant biological characteristics of Huizhuo Tanzu type atherosclerosis

indicating that animal models were successfully made.