Objective:To study the effects of Rubia cordifolia L. and Charred R. cordifolia on coagulation-fibrinolytic system in normal Wistar rats and their mechanism of stasis-resolving hemostatic based on their pharmacological action. Method: Wistar rats were randomly divided into blank control group(normal saline

10 mL·kg-1)

R. cordifolia low-dose (5.0 g·kg-1) and high-dose (10.0 g·kg-1) groups

Charred R. cordifolia low-dose (5.0 g·kg-1) and high-dose (10.0 g·kg-1) groups and positive control group p-aminomethylbenzoic acid

(0.6 g·kg-1) ig

one time a day for 7 days. The blood in abdominal aorta was taken and then the plasma was separated in the eighth day. The activities of plasma tissue-type plasminogen activator (t-PA)

plasminogen activator inhibitor (PAI) and plasminogen (PLG) in plasma were measured by ELISA method. Result: Compared with the blank control group

both the low-dose and high-dose group of R. cordifolia can increase t-PA activity

the high-dose group of R. cordifolia can increase t-PA activity more obviously (P＜0.01). The high-dose group of Charred Rubia cordifolia L. can also increase t-PA activity (P＜0.05) but the low-dose group of Charred Rubia cordifolia L. cannot. In addition

the high-dose group of Charred R.cordifolia's effect on t-PA activity is smaller than the high-dose group of R. cordifolia The effects of R. cordifolia and Charred R. cordifolia on the PAI and PLG activity are all not significant regardless of their dosage. Conclusion: Both the low-dose and the high-dose group of R. cordifolia can remarkably affect fibrinolytic system to play its role on stasis-resolving hemostatic through increasing t-PA activity. Charred R. cordifolia can also affect fibrinolytic system while it may be the comprehensive effects of many enzymes. R. cordifolia and Charred R. cordifolia have different pharmacological effects on fibrinolytic system. Thereby

they have different uses in clinical practice.