Objective: To prepare affinity medium with four effective ingredients of ginsenoside Rg1

ginsenoside Rb1

ophiopogonin DT-13 and schisandrin from Shengmai powder as ligands. Method: Affinity medium was prepared by coupling with four effective ingredients and epoxy-activated-sepharoseTM6B(EAS 6B).A HPLC-DAD method for simultaneous separation and determination of four ingredients was established

with coupling rate as index

effect of feeding rate

reaction time and temperature on coupling reaction was investigated by orthogonal test

then optimized preparation technology. Result: Optimum preparation technology was as following:reaction time 24 h

reaction temperature 25℃

added 10 times the amount of active ingredients ligands.Four ingredients could coupling with EAS 6B

coupling rate with four ingredients and affinity medium was ginsenoside Rg1 of 27.40%

ginsenoside Rb1 of 22.30%

ophiopogonin DT-13 of 50%

schizandrin of 24.50%. Conclusion: We successfully prepared affinity medium with four effective compounds deprived from Shengmai powder as ligands.It provided technical means to further identify target of this compound and some references for preparing affinity medium of other multiple natural compounds with similar active groups.