CHEN Zhi, Chen Kai, TIAN Jing-zhen. Optimization of Purification Technology for Polysaccharides from by Freezing and Thawing-Enzymolysis-Membrane Separation Composite Technology[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(14): 42-44.
DOI:
CHEN Zhi, Chen Kai, TIAN Jing-zhen. Optimization of Purification Technology for Polysaccharides from by Freezing and Thawing-Enzymolysis-Membrane Separation Composite Technology[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(14): 42-44. DOI: 10.11653/syfj2013140042.
Optimization of Purification Technology for Polysaccharides from by Freezing and Thawing-Enzymolysis-Membrane Separation Composite Technology
Objective: To optimize purification technology of polysaccharides from Isatis indigotica by freezing and thawing-enzymolysis-membrane separation composite technology. Method: With the content of polysaccharides from I. indigotica and remaining protein as indexes
based on single factor tests
orthogonal test was adopted to investigate effect of hydrolysis temperature
reaction time and enzyme dosage on purification technology. Result: Optimal purification process was as following:repeated freezing and thawing three times of water extract liquid of polysaccharides from I. indigotica residue
filtered
added protease 2 with dosage of 35 000 U·g-1
enzymatic temperature for 35℃
hydrolysis time of 3 h
then ultrafiltrated with hollow fiber components of interception molecular mass 30 kD. Conclusion: This optimized technology was stable and feasible
freezing and thawing-enzymolysis-membrane separation composite technology could be used to purify polysaccharides from I. indigotica.
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