ZHANG Xiao-li, LI Kun. Purification and Thrombolytic Activity of Polypeptide from [J]. Chinese journal of experimental traditional medical formulae, 2013, 19(14): 53-55.
DOI:
ZHANG Xiao-li, LI Kun. Purification and Thrombolytic Activity of Polypeptide from [J]. Chinese journal of experimental traditional medical formulae, 2013, 19(14): 53-55. DOI: 10.11653/syfj2013140053.
Purification and Thrombolytic Activity of Polypeptide from
Objective: To purify polypeptide from Eupolyphaga sinensis and investigate its thrombolytic activity. Method: With thrombolytic activity as index
polypeptide was obtained by bionic enzymatic
which was purified by Sephadex G-25
SP Sephadex C-25 and RP-HPLC C18 semi-preparation column.Thrombolytic activity
peptide content and relative molecular weight of target component were determined. Result: Thrombolytic activity of target polypeptide component was 814 430 U·mg-1
peptide content was 95.7% and relative molecular weight was in the range of 3 211-3 547. Conclusion: Polypeptide from E. sinensis could be prepared by bionic enzymatic
and a component of polypeptide with strong thrombolytic activity could be obtained through separation and purification.