Objective: To develop based formulation of Cervus elaphus amino acid oral liquid. Method: Selected two C. elaphus powder from the same origin

divided into the biomimetic group and the ultrasound group according to different extraction methods.The content of amino acid of different groups was compared

and SIMCA-P statistical software was adopted to do principal component analysis. Result: There was a significant difference between samples from two groups

especially methionine

proline

arginine

threonine

isoleucine

phenylalanine and valine. Formulation of core amino acids group was as following:methionine 0.96%

proline 11.50%

arginine 8.29%

threonine 2.64%

isoleucine 1.71%

phenylalanine 2.62% and valine 3.46%;Formulation of based amino acids group was aspartic acid 7.39%

serine 4.07%

glutamic acid 13.31%

glycine 21.72%

alanine 10.30%

leucine 4.64%

tyrosine 0.97%

lysine 5.07%

histidine 1.36%. Conclusion: There are some differences in amino acid proportion between the natural state and the digestion state.With amino acids proportion as basis

this developed C. elaphus amino acid oral liquid could get better tonic effect of C. elaphus.