Objective: To establish an HPLC method for determining the content of hyperoside

quercitrin and quercetin in Rhododendron Primulaeflorum

and to lay the foundation for the quality control and/or quality evalution of R. primulaeflorum. Method: A high-performance liquid chromatography equipped a Welch Ultimate XB-C18 (4.6 mm×250 mm

5 μm) column with UV detection was used. The mobile phase consisted of acetonitrile and methanol (5:1)(A) and 0.1% formic acid in water (B) with gradient elution. The column temperature was kept at 30℃ and the flow rate was 1.0 mL·min-1. The detection wavelength was set at 350 nm. Result: The good separation of three compounds was achieved within 70 min. The linear range of hyperoside

quercitrin and quercetin were 4.41-88.20 mg·L-1 (r=0.999 6)

2.94-58.80 mg·L-1 (r=0.999 6)

1.485-29.70 mg·L-1 (r=0.999 6) respectively. The average recovery were 103.37%(RSD 1.67%)

98.07%(RSD 1.4%)

100.19%(RSD 1.20%) respectively. Conclusion: This method is simple

accurate with good reproducibility and stability. It can be used as effective methods for quality control of R. primulaeflorum.