Determination of Geniposide and Physcion in Compound Longli Capsule by HPLC

DU Cheng-zhi; TAN Jian-ning; MA Wen-Fang

doi:10.11653/syfj2013140135

您当前的位置：

首页 >

文章列表页 >

Determination of Geniposide and Physcion in Compound Longli Capsule by HPLC

更新时间：2024-01-04

- Determination of Geniposide and Physcion in Compound Longli Capsule by HPLC
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 19, Issue 14, Pages: 135-138(2013)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.11653/syfj2013140135
  CLC：
- Published：2013
- 稿件说明：
移动端阅览
DU Cheng-zhi, TAN Jian-ning, MA Wen-Fang. Determination of Geniposide and Physcion in Compound Longli Capsule by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(14): 135-138.
DOI：

DU Cheng-zhi, TAN Jian-ning, MA Wen-Fang. Determination of Geniposide and Physcion in Compound Longli Capsule by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(14): 135-138. DOI： 10.11653/syfj2013140135.

摘要

目的: 建立复方龙脷胶囊中栀子苷和大黄素甲醚的测定方法。方法: 采用Waters Symmetry C18色谱柱(4.6 mm×250 mm

5 μm)

流动相分别为乙腈-水(15:85)、甲醇-水(95:5)

流速1.0 mL·min-1

检测波长分别为238

254 nm

柱温25℃。结果: 栀子苷、大黄素甲醚分别在0.493 2~2.959 2 μg (r=0.999 5)和0.063 4~0.380 4 μg (r=1.000 0) 线性关系良好

平均加样回收率分别为100.4%

101.3%

RSD分别为1.9%

1.7%。结论: 方法简便可行

结果准确

重复性好

可用于复方龙脷胶囊中栀子苷和大黄素甲醚的含量测定。

Abstract

Objective: To establish a HPLC method for the determination of Geniposide and Physcion in Compound Longli Capsule. Method: Waters Symmetry C18 analytical column (4.6 mm×250 mm

5 μm) was used. The mobile phase was consisted of acetonitrile-water (15:85) and methanol-water (95:5) respectively;the flow rate was 1.0 mL·min-1

UV detection wavelength was set at 238

254 nm respectively. The column temperature was maintained at 25℃. Result: Under the chromatographic conditions used

the calibration curve of geniposide and physcion was linear in the range of 0.493 2-2.959 2 μg (r=0.999 5) and 0.063 4-0.380 4 μg (r=1.000 0) respectively. The average recoveries was 100.4% and 101.3% with RSD 1.9% and 1.7% respectively. Conclusion: The method is accurate

effective and feasible. It can be used for the determination of the content of geniposide and physcion contained in Compound Longli capsule.

关键词

Keywords

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Determination of Geniposide in Wujunzhidan Tablet by HPLC

Determination of Geniposide in Biyuanshu Granules by HPLC

Determination of Geniposide in Yinzhi Oral Solution by HPLC

HPLC Determination of Geniposide, Andrographolide and Dehydroandrographolide in Lianzhi Qinghuo Capsules

Related Author

ZHOU Hai

MO Jing-chang

ZHANG Pei-qin

SU Song-bai

ZHANG Jian-ling

WAN Jun

LI Xiang

LIU Gui-yang

Related Institution

The First Affiliated Hospital of Guiyang College of Traditional Chinese Medicine

Shuguang Hospital， Institute of Liver Diseases， Key Laboratory of Liver and Kidney Disease （Ministry of Education）， Shanghai Key Laboratory of Traditional Chinese Clinical Medicine，Shanghai University of Traditional Chinese Medicine （TCM）

Institute of Chinese Materia Medica， Institute of Interdisciplinary Integrative Medicine， Shanghai University of TCM

Key Laboratory of Modern Preparation of TCM Ministry of Education， Jiangxi University of TCM

School of Pharmacy，Jiangxi University of Traditional Chinese Medicine

AI问答

Postal code：100700
Tel：010-84076882 Email：syfjx_2010@188.com
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备11006657号-10 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰