HPLC Quantitative Determination of Hyperin in Shenqiqiangjing Capsule

TIAN Yuan-chun; LIU Qian; WEI Shui-lin; BIN Bin

doi:10.11653/syfj2013140149

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HPLC Quantitative Determination of Hyperin in Shenqiqiangjing Capsule

更新时间：2024-01-04

- HPLC Quantitative Determination of Hyperin in Shenqiqiangjing Capsule
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 19, Issue 14, Pages: 149-151(2013)
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- DOI：10.11653/syfj2013140149
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- Published：2013
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TIAN Yuan-chun, LIU Qian, WEI Shui-lin, et al. HPLC Quantitative Determination of Hyperin in Shenqiqiangjing Capsule[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(14): 149-151.
DOI：

TIAN Yuan-chun, LIU Qian, WEI Shui-lin, et al. HPLC Quantitative Determination of Hyperin in Shenqiqiangjing Capsule[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(14): 149-151. DOI： 10.11653/syfj2013140149.

摘要

目的: 建立参杞强精胶囊中金丝桃苷含量的高效液相色谱测定方法。方法: 采用Diamonsil C18色谱柱(4.6 mm×250 mm

5 μm)

流动相乙腈-0.1%磷酸溶液(17:83)

流速1.0 mL·min-1

检测波长350 nm

柱温31℃

进样量20 μL。结果: 金丝桃苷与样品中其他组分分离效果好。金丝桃苷的线性范围为0.120 1~1.201 6 μg (r=0.999 9)

平均加样回收率(n=6) 99.12%(RSD 1.90%);供试品溶液在24 h内稳定。结论: 方法灵敏度高

操作简便

准确可靠

可用于参杞强精胶囊中金丝桃苷的含量测定。

Abstract

Objective: To develop an HPLC method for determination of hyperin in Shenqiqiangjing Capsule. Method: Diamonsil C18(4.6 mm×250 mm

5 μm) column was adopted

the mobile phase consisted of acetonitrile-0.1% phosphatic acid (17:83) at a flow rate of 1.0 mL·min-1

the detection wavelength was set at 350 nm and column temperature was set at 31℃. The injection volume was 20 μL. Result: The linear range was 0.120 1-1.201 6 μg (r=0.999 9)

the average recovery (n=6) was 99.12%(RSD 1.90%). The test solution was stable in 24 h. Conclusion: The method is sensitive

convenient and accurate

which can be applied in the determination of Shenqiqiangjing Capsule.

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