Simultaneous Determination of Uridine and Adenosine in Allii Tuberosi Semen by RP-HPLC

SHENG Kang-mei; YANG Li-xin; ZHAO Hai-yu; SI Nan; YANG Jian; WANG Hong-jie

doi:10.11653/syfj2013140152

您当前的位置：

首页 >

文章列表页 >

Simultaneous Determination of Uridine and Adenosine in Allii Tuberosi Semen by RP-HPLC

更新时间：2024-01-04

- Simultaneous Determination of Uridine and Adenosine in Allii Tuberosi Semen by RP-HPLC
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 19, Issue 14, Pages: 152-154(2013)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.11653/syfj2013140152
  CLC：
- Published：2013
- 稿件说明：
移动端阅览
SHENG Kang-mei, YANG Li-xin, ZHAO Hai-yu, et al. Simultaneous Determination of Uridine and Adenosine in Allii Tuberosi Semen by RP-HPLC[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(14): 152-154.
DOI：

SHENG Kang-mei, YANG Li-xin, ZHAO Hai-yu, et al. Simultaneous Determination of Uridine and Adenosine in Allii Tuberosi Semen by RP-HPLC[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(14): 152-154. DOI： 10.11653/syfj2013140152.

摘要

目的: 建立同时测定韭菜子中的尿苷和腺苷的RP-HPLC含量测定方法。方法: CNW C18-WP色谱柱(4.6 mm×250 mm

5 μm)

流动相为A(水)-B(乙腈)

梯度洗脱(0~20 min

100%~80%A

0%~20%B);检测波长260 nm。结果: 尿苷和腺苷分别在9.56~95.60 ng和10.20~102.0 ng与峰面积呈良好线性关系

r分别为0.999 7

0.999 6;平均回收率分别为98.04%

101.99%

RSD分别为0.99%

1.44%。结论: 该方法简便易行

具有良好的重复性和稳定性

可用于韭菜子质量控制。

Abstract

Objective: To establish the RP-HPLC quantitative determination method of uridine and adenosine in Allii Tuberosi Semen. Method: CNW C18-WP column (4.6 mm×250 mm

5 μm) was applied with water (A)-acetonitrile (B) as mobile phase. The gradient elution condition was 0-20 min

0%-20% B and the detection wavelength was set at 260 nm. Result: Uridine and adenosine revealed good linear relation from 9.56 to 95.60 ng and from 10.20 to 102.0 ng respectively with correlation coefficient (r) of 0.999 7 and 0.999 6.The average recoveries of them were 98.04% and 101.99% with RSD of 0.99% and 1.44%. Conclusion: This method was simple and accurate with good repeatability and stability

which was suitable for the quality control of Allii Tuberosi Semen.

关键词

Keywords

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Establishment of Quality Control Method in Production of Jinshuibao Capsules

Simultaneous Determination of Four Active Components in Radix Isatidis by HPLC with Double-wavelength Switch

Determination of Content of Uridine,Guanosine and Adenosine in by UPLC

Determination of Uridine, Guanosine and Adenosine Contents in Corydalis Rhizoma from Different Regions and Commercial Grades

Optimization of Extraction Technology for Total Nucleosides from by Orthogomal Test

Related Author

Xue-ting ZHANG

Cheng-zan ZHONG

Ming YANG

Sha CHEN

XU Gui-hua

ZHANG Ji-huan

QIAO Jian-wei

SU Rui-qiang

Related Institution

Key Laboratory of Identification and Safety Evaluation of Chinese Medicine, Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences

Jiangxi Guoyao Co. Ltd.

Lunan Pharmaceutical Group Co.,Ltd.

State Key Laboratoryof Generic Pharmaceutical Technology for Chinese Traditional Medicine

School of Chinese Materia Medica, Beijing University of Chinese Medicine

AI问答

Postal code：100700
Tel：010-84076882 Email：syfjx_2010@188.com
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备11006657号-10 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰