Direct Determination of 2 Underivatized Amino Acids in Compound Gancaosuanan Injection by HPLC-ELSD

ZHANG Jian-ye; FENG Xiang-dong

doi:10.11653/syfj2013140161

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Direct Determination of 2 Underivatized Amino Acids in Compound Gancaosuanan Injection by HPLC-ELSD

更新时间：2024-01-04

- Direct Determination of 2 Underivatized Amino Acids in Compound Gancaosuanan Injection by HPLC-ELSD
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 19, Issue 14, Pages: 161-163(2013)
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- DOI：10.11653/syfj2013140161
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- Published：2013
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ZHANG Jian-ye, FENG Xiang-dong. Direct Determination of 2 Underivatized Amino Acids in Compound Gancaosuanan Injection by HPLC-ELSD[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(14): 161-163.
DOI：

ZHANG Jian-ye, FENG Xiang-dong. Direct Determination of 2 Underivatized Amino Acids in Compound Gancaosuanan Injection by HPLC-ELSD[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(14): 161-163. DOI： 10.11653/syfj2013140161.

摘要

目的: 用HPLC-ELSD法直接测定复方甘草酸铵注射液中2种未衍生氨基酸的含量。方法: 使用Agilent-ODS C18(4.6 mm×250 mm

5μm)色谱柱

流动相A(乙腈)-B(5.0 mmol·L-1七氟丁酸的0.7%三氟醋酸溶液)

梯度洗脱(0~5 min

0%A;5~10 min

15%A;10~25 min

35%A)

ELSD漂移管温度80℃

氮气流量2.0 L·min-1

流速0.8 mL·min-1

柱温35℃

进样10 μL。结果: 甘氨酸进样量在4.858~29.145 μg

盐酸半胱氨酸进样量在0.386~2.316 μg时

其对数值与峰面积对数值线性关系良好

回收率分别为97.43%

96.99%。结论: 测定方法简便

结果准确

可用于复方甘草酸铵注射液中2种氨基酸的测定。

Abstract

Objective: To determine 2 underivatized amino acids in Compound Gancaosuanan Injection with HPLC-ELSD. Method: The analysis was carried out on a Agilent-ODS C18 (4.6 mm×250 mm

5 μm) column. The mobile phase was acetonitrile (A)-5.0 mmol·L-1 heptafluorobutyric acid in 0.7% trifluoroacetic acid solution (B) in gradient elution mode(0-5 min

0%A;5-10 min

15% A;10-25 min

35%A). Drift tube temperature was 80℃

gas flow rate was 2.0 L·min-1. The flow rate was 0.8 mL·min-1. Column temperature was kept at 35℃ and the inject volume was 10 μL. Result: The glycine and cysteine hydrochloride were linear in the range of 4.858-29.145 μg and 0.386-2.316 μg. The average recovery was 97.43%

96.99% respectively. Conclusion: The method is convenient and accurate

which can be used to determine 2 amino acids in Compound Gancaosuanan Injection.

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