Objective: To study effects of Persicae Semen on apoptosis and protein expression of endothelial cell in cold stagnation and blood stasis syndrome (HS) and heat stagnation and blood stasis syndrome (RS) rats. Method: Eighty rats were randomly divided into 8 groups including normal control group of HS (HN)

model group of HS (HM)

Persicae Semen treatment group of HS (HT)

Chuanxiong Rhizoma control group of HS (HC)

normal control group of RS (RN)

model group of RS (RM)

Persicae Semen treatment group of RS (RT)

Salviae Miltiorrhizae Radix et Rhizoma control group of RS (RD). To establish the two rat models by freezing and injection of carrageenan solution. Extracts of Persicae Semen

Chuanxiong Rhizoma and Salviae Miltiorrhizae Radix et Rhizoma were given to the corresponding group rats.Severn days later

Four hundred organs derived from the eighty rats were detected by histopathology analysis (HE

IHC and TUNEL). The indexes included growth factor VEGF

apoptotic inhibitor Bcl-2

apoptotic induced protein p53 and apoptotic number of vascular endothelial cell (VEC). Result: ①The corresponding blood stasis syndromes appeared in HM and RM rats

while improvements of the two syndromes appeared in HT and RT rats. ② Decreased VEGF expression appeared just in RT rats(P<0.05)

while it did not change in HT rats. Increased Bcl-2 expression appeared just in RT rats(P<0.05)

while it did not change in HT rats. p53 expression did not change in both HT and RT rats. ③Apoptosis did not change in both HT and RT rats. Conclusion: Persicae Semen proved the blood circulation and suppressed apoptosis of endothelial cell in blood stasis rats

it might be caused Persicae Semen decreased VEGF and increased Bcl-2 expression of endothelial cells