Neuroprotective Effect of Shengmai Injection on Expression of Tissue Factor and Related Signal Pathways in Mice with Cerebral Ischemia-reperfusion Injury
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Neuroprotective Effect of Shengmai Injection on Expression of Tissue Factor and Related Signal Pathways in Mice with Cerebral Ischemia-reperfusion Injury
Chinese Journal of Experimental Traditional Medical FormulaeVol. 19, Issue 14, Pages: 194-199(2013)
YANG Hao-peng, LI Long, CHAI Cheng-zhi, et al. Neuroprotective Effect of Shengmai Injection on Expression of Tissue Factor and Related Signal Pathways in Mice with Cerebral Ischemia-reperfusion Injury[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(14): 194-199.
DOI:
YANG Hao-peng, LI Long, CHAI Cheng-zhi, et al. Neuroprotective Effect of Shengmai Injection on Expression of Tissue Factor and Related Signal Pathways in Mice with Cerebral Ischemia-reperfusion Injury[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(14): 194-199. DOI: 10.11653/syfj2013140194.
Neuroprotective Effect of Shengmai Injection on Expression of Tissue Factor and Related Signal Pathways in Mice with Cerebral Ischemia-reperfusion Injury
Objective: To investigate the neuroprotective effect of Shengmai injection (SMI) on the expression of tissue factor (TF) and related signal pathways in mice with cerebral ischemia-reperfusion injury. Method: Mice were randomly divided into 5 groups as following:sham operation group
ischemia-reperfusion(I/R) group
SMI groups at three doses (5.68
2.84
1.42 g·kg-1). Right middle cerebral artery was occluded by inserting a thread through internal carotid artery for 1 h. The infarct volume and neurological scores were evaluated after 24 h reperfusion. Western blotting was used to determine the expression of tissue factor (TF)
nuclear factor-kappa B (NF-κB) subunit p65
Akt
and their phosphorylation after 1 h reperfusion. Result: Compared with I/R group
SMI could significantly reduce the infarct volume and improve the neurological deficits in a dose-dependent manner. SMI also remarkably down-regulated the expression of TF
inhibited the activation of NF-κB induced by ischemia-reperfusion and the phosphorylation of Akt in mice. Conclusion: Shengmai injection
at certain dosage range
elicits potent protection against cerebral ischemia/reperfusion injury
which may partly be due to inhibiting TF expression and its related pathways.