Objective: To observe the anti-inflammatory and analgesic effects of Xiaoaishu(XAS) and explore it's mechanism. Method: The KM mice were randomly divided into model group

aspirin group(0.2 g·kg-1) and XAS groups (0.375

0.75

1.5 g·kg-1) ig

for 7 d. Carrageenan-induced paw swelling and dimethylbenzene-induced pinna swelling in mice were used to evaluate the anti-inflammatory effects. The analgesic effects of XAS were tested by counting writhing frequency induced by acetic acid and measuring the latent period of licking hind foot with the hot plate method in mice.The levels of tumor necrosis factor-α(TNF-α) and interleukin-1β(IL-1β) mRNA was determined by real time RT-PCR. Result: XAS significantly suppressed the dimethylbenzene-induced mice auricle swelling and Carrageenan-induced mice paw edema(P<0.05)

XAS significantly reduced the acetic acid-induced writhing frequency and pain threshold in mice(P<0.05)

real-time RT-PCR showed that XAS could decrease the level of TNF-α and IL-1β mRNA of inflammatory tissue in mice (P<0.05). Conclusion: XAS have evident anti-inflammatory and analgesic effects

the mechanism may be related with the inhibition of the expression of TNF-α and IL-1β mRNA in the carrageenen-induced mice paw.