Objective: To study the effects and mechanism of momordica grosvenori flavones (MGF) on myocardial energy metabolism and expression of peroxisome proliferators-activated receptor α(PPARα) mRNA in exercise rats. Method: Fifty healthy male Sprague-Dawley rats were randomly divided into five groups (n=10):quiet control group (NC)

exercise control group (ME)

exercise+ig low dose flavones group (MGFL)

ig moderate dose flavones group (MGFM) and intragastric gavage (ig) high dose flavones group (MGFH). All rats in administrated groups were intragastric gavaged in profession once time at half an hour before exercise. The administration was continued for 6 d/week in total 6 weeks at the dose of 100

200

400 mg·kg-1 in low

medium and high dose group respectively

while the rats in unadministrated groups were treated by physiological saline solution according to the same dose. The rats were killed immediately by decapitation at the end of training. The activities of creatine kinase (CK)

lactate dehydrogenase (LDH) and succinate dehydrogenase (SDH) in myocardial tissue were measured according the corresponding kit method respectively. The expression of PPARαmRNA in myocardial tissue was determined reverse transcription polymerase chain reaction (RT-PCR). Result: The activity of myocardial CK was decreased in rat by exhaustive swimming exercise. It was significantly lower in exercise control group than that in quiet control group (P<0.01). There was significant difference in low dose MGF group compared with quiet control group (P<0.05). The activity of myocardial CK was obviously higher in MGF groups than that in exercise control group (P<0.01). The activity of LDH was significantly lower in exhaustive swimming groups than that in quiet control group (P<0.01). It was higher in each MGF group than that in exercise control group (P<0.05 or P<0.01). The activity of SDH was obviously lower in exhaustive swimming groups than that in quiet control group (P<0.01). It was lower in low dose MGF group than that in quiet control group (P<0.05)

while it was higher in moderate

and high dose MGF groups than that in exercise control group (P<0.05). The expression of PPARα mRNA was obviously lower in exercise control group than that in quiet control group (P<0.01). It was higher in MGFL group than that in exercise control group (P<0.05). The expression of PPARα mRNA in MGFM and MGFH groups were significantly higher than that in exercise control group (P<0.01). Conclusion: Supplement of momordica grosvenori flavones could improve the activities of energy metabolic enzymes CK

LDH and SDH

and upregulate the expression of PPARα mRNA. This has a good role in improving the energy metabolism and protecting the myocardial tissue from overuse exercise injury.