GAO Yuan, CHEN Gui-min, LIANG Zhen-yu, et al. Impact of Qigong Kangxian Prescription on Smad2/3 and Smad7 in Hepatic Fibrosis Rat[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(14): 229-232.
DOI:
GAO Yuan, CHEN Gui-min, LIANG Zhen-yu, et al. Impact of Qigong Kangxian Prescription on Smad2/3 and Smad7 in Hepatic Fibrosis Rat[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(14): 229-232. DOI: 10.11653/syfj2013140229.
Impact of Qigong Kangxian Prescription on Smad2/3 and Smad7 in Hepatic Fibrosis Rat
Objective: To explore the impact of Qigong Kangxian prescription(QF) on Smad 2/3
Smad7 in hepatic fibrosis rats. Method: Sixty SD rats randomly divided into 6 groups
in addition to the normal control group
the other groups were intraperitoneally injected with 0.5 mL pig's serum twice per week for 8 weeks
the rat hepatic fibrosis model was induced. Rats were given corresponding herbal medicines. After the end of experiment
serum samples were collected. Determination of the content of transforming growth factor was carried out by using ELISA method. Sections of liver tissue was fixed in 10% formaldehyde solution
and then embedded in paraffin
Under the light microscope. Expression of liver tissue TGF-β1
Smad2
Smad3
Smad7 was detected by immunohistochemical method. Result: In the Content of TGF——β1 and expression of liver tissue TGF-β1
Smad2
Smad3 and Smad7 the significant differences were found in administration groups compared with the model group(P<0.01). Conclusion: QF can suppress hyperplasy of hepatic fibrous connective tissue
lessen hepatic injury through decreasing Smad 2/3 expression and increase Smad7 expression.