Objective: Effect of tanshinone ⅡA on homocysteine (HCY) induced proliferation of vascular smooth muscle cells (VSMC) of endoplasmic reticulum stress (ERS) gene of immunoglobulin heavy chain binding protein (Bip) expression

to explore the mechanism of tanshinone ⅡA in atherosclerosis. Method: To establish the proliferation of rabbit VSMC model induced by HCY

incubated with different concentration of tanshinone ⅡA

Annexin/PI double staining

in situ nick end labeling (TUNEL) method to detect apoptosis rate

to observe the morphology of cell apoptosis; real-time fluorescent quantitative PCR detection the expression of Bip gene. Result: Tanshinone ⅡA can significantly promote the apoptosis of rabbit VSMC

cell apoptosis rate compared with HCY group (P<0.01)

and there was a dose dependent; expression levels of tanshinone ⅡA group Bip mRNA were significantly increased

compared with the HCY group had significant difference (P<0.05

P<0.01). Conclusion: Expression of tanshinone ⅡA up-regulation of Bip gene

amplified ERS signal

induce apoptosis of rabbit VSMC

which may be one of the mechanisms of atherosclerosis.