Evaluation of Three Preparation Technologies for  Extract by HPLC Fingerprint

LI Xiao-kun; LIU Jiong; Zhang Jie; ZHANG Hua-feng; LIU Fu-gang; YANG Yun

doi:10.11653/syfj2013150010

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Evaluation of Three Preparation Technologies for Extract by HPLC Fingerprint

更新时间：2024-01-04

- Evaluation of Three Preparation Technologies for Extract by HPLC Fingerprint
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 19, Issue 15, Pages: 10-13(2013)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.11653/syfj2013150010
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- Published：2013
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LI Xiao-kun, LIU Jiong, Zhang Jie, et al. Evaluation of Three Preparation Technologies for Extract by HPLC Fingerprint[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(15): 10-13.
DOI：

LI Xiao-kun, LIU Jiong, Zhang Jie, et al. Evaluation of Three Preparation Technologies for Extract by HPLC Fingerprint[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(15): 10-13. DOI： 10.11653/syfj2013150010.

摘要

目的: 比较3种贯叶连翘提取物制备工艺的稳定性与重复性。方法: 建立提取物的HPLC指纹图谱

对不同批次提取物进行相似度分析、聚类分析和模式识别。结果: 3种工艺均具有良好的稳定性与重复性

且酶解后优于酶解前

纯化后优于纯化前

以酶解后经大孔树脂LSA-10纯化所得提取物的制备工艺为最佳。不同批次提取物的镜像度较高

具有良好的相似性(>0.9)。结论: HPLC指纹图谱可用于同类提取物的质量控制和制备工艺评价。

Abstract

Objective: To compare stability and reproducibility of three preparation processes for Hypericum perforatum extract. Method: HPLC fingerprint of H. perforatum extract was established

and different batches of extract were analyzed by similarity analysis

cluster analysis and pattern recognition. Result: These three processes had good stability and reproducibility

and enzymatic hydrolysis was superior to before enzymatic hydrolysis

purification was superior to before purification

optimum preparation technology was purified by LSA-10 macroporous resin after enzymatic hydrolusis.Mirror degree of different batches of extract was high with good similarity(>0.9). Conclusion: HPLC fingerprint analysis method could be adopted for quality control and process evaluation of similar extract.

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Related Institution

Institute of Chinese Materia Medica，China Academy of Chinese Medical Sciences

Medical School， Lijiang Culture and Tourism College

Key Laboratory of Modern Preparation of Traditional Chinese Medicine （TCM），Ministry of Education，Jiangxi University of Chinese Medicine

Chengdu University of TCM

Medical School of Lijiang Culture and Tourism College

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