LUO Sai-sai, HAO Ting, ZHAO Gui-qin. Optimization of Enrichment and Purification Technology for Total Iridoid Glycosides from by Macroporous Resin[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(15): 13-16.
DOI:
LUO Sai-sai, HAO Ting, ZHAO Gui-qin. Optimization of Enrichment and Purification Technology for Total Iridoid Glycosides from by Macroporous Resin[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(15): 13-16. DOI: 10.11653/syfj2013150013.
Optimization of Enrichment and Purification Technology for Total Iridoid Glycosides from by Macroporous Resin
Objective: To optimize enrichment and purification technology of total iridoid glycosides from Jasminum officinale by macroporous resin. Method: With oleuropein as index
its content was determined by HPLC. Types of macroporous resin were optimized by static adsorption-elution test
enrichment and purification technology was investigated by orthogonal test and single-factor tests. Result: XDA-16 macroporous resin showed better effect than others
optimum purification technology was as following:the concentration of sample liquid 0.08 g·mL-1
height-diameter ratio of 1:3
absorption velocity 2 BV·h-1
dosage of sample liquid of 0.7 g·g-1
washed impurity with 8 BV water
then eluted wtih 4 BV 70% ethanol at 2 BV·h-1
collected eluent. Conclusion: This optimized technology was stable and feasible.XDA-16 macroporous resin had a good enrichment and purification effect on total iridoid glycosides from J. officinale.
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