WANG Yan-yan, SUN Hai-jiao, WANG Zhi-bin, et al. Optimization of Purification Technology for Total Steroids of Withanolides from Effective Part of [J]. Chinese journal of experimental traditional medical formulae, 2013, 19(15): 17-19.
DOI:
WANG Yan-yan, SUN Hai-jiao, WANG Zhi-bin, et al. Optimization of Purification Technology for Total Steroids of Withanolides from Effective Part of [J]. Chinese journal of experimental traditional medical formulae, 2013, 19(15): 17-19. DOI: 10.11653/syfj2013150017.
Optimization of Purification Technology for Total Steroids of Withanolides from Effective Part of
Objective: To optimize separation and purification technology for total steroids of withanolides from effective part of trearment psoriasis in Datura metel. Method: With content and purity of total steroids as indexes
optimum resin model was screened by comparing adsorption properties and elution ability of different macroporous resins.Single factor tests were adopted to investigate effect of loading capacity
type of eluent
elution rate and other parameters on purification technology. Result: D941 macroporous resin was selected
its optimum purification technology was as following:ratio of sample mass to macroporous resin<7.5 g·L-1
pH of sample liquid 7
eluted with 3 BV water at elution flow speed of 6 BV·h-1.After purified
purity of total steroids was more than 90%. Conclusion: This optimized purification technology was simple
stable and feasible with good purification effect.
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GUO Hong-wei
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Guangdong Engineering and Technology Research Center of Standardized Processing of Chinese Materia Medica Decoction Pieces,School of Traditional Chinese Medicine (TCM), Guangdong Pharmaceutical University
Key Laboratory of Basic and Applied Research of Northern Medicine,Ministry of Education,Heilongjiang Key Laboratory of Pharmacodynamic Material Basis of TCM and Natural Medicine,Heilongjiang University of Chinese Medicine
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