Comparison of Extraction Methods for Protein in Tissue of

GAO Fang

doi:10.11653/syfj2013150032

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Comparison of Extraction Methods for Protein in Tissue of

更新时间：2024-01-04

- Comparison of Extraction Methods for Protein in Tissue of
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 19, Issue 15, Pages: 32-34(2013)
- 作者机构：
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- DOI：10.11653/syfj2013150032
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- Published：2013
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GAO Fang. Comparison of Extraction Methods for Protein in Tissue of [J]. Chinese journal of experimental traditional medical formulae, 2013, 19(15): 32-34.
DOI：

GAO Fang. Comparison of Extraction Methods for Protein in Tissue of [J]. Chinese journal of experimental traditional medical formulae, 2013, 19(15): 32-34. DOI： 10.11653/syfj2013150032.

摘要

目的: 比较雪莲组织中蛋白质的提取方法。方法: 采用酚法、TCA/丙酮法、尿素法提取雪莲组织中蛋白质

所得蛋白质进行一维SDS-PAGE和双向电泳检测

通过比较电泳图谱确定雪莲组织中蛋白质的最佳提取方法。结果: 酚法提取的雪莲组织蛋白质杂质量最少、纯度最高

一维SDS-PAGE和双向电泳图谱显示其具有较多的蛋白质点

且蛋白质种类较多。结论: 3种提取方法均有一定的蛋白质损失

建议采用酚法从雪莲组织中提取蛋白质

为雪莲蛋白质组学研究提供参考。

Abstract

Objective: To comparative analyze different extraction methods for protein in tissue of Saussurea involucrate. Method: Protein in tissue of S. involucrate was extracted by phenol method

TCA/acetone method and urea method

prepared protein was detected by one-dimensional SDS-PAGE and two-dimensional electrophoresis

then optimum extraction method for protein in tissue of S. involucrate was determined by comparing electrophoretic patterns. Result: Phenol extraction method was optimal with the highest purity and the least impurities

one-dimensional SDS-PAGE and two-dimensional electrophoresis showed that there were more protein spots and species. Conclusion: Three extraction methods had some protein loss

but phenol method was the best for extracting protein from tissue of S. involucrate

this study could provide reference for investigating proteomics of S. involucrate.

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