Simultaneous Determination of Polyphyllin Ⅰ,Ⅱ, Ⅵ,Ⅶ and Hyperoside in Chonglou Kegan Capsule

YIN Xing-bin; LIN Long-fei; CAO Sa-li; ZHANG Hui; ZHANG Jin; QI Juan-juan; NI Jian

doi:10.11653/syfj2013150058

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Simultaneous Determination of Polyphyllin Ⅰ,Ⅱ, Ⅵ,Ⅶ and Hyperoside in Chonglou Kegan Capsule

更新时间：2024-01-04

- Simultaneous Determination of Polyphyllin Ⅰ,Ⅱ, Ⅵ,Ⅶ and Hyperoside in Chonglou Kegan Capsule
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 19, Issue 15, Pages: 58-61(2013)
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- DOI：10.11653/syfj2013150058
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- Published：2013
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YIN Xing-bin, LIN Long-fei, CAO Sa-li, et al. Simultaneous Determination of Polyphyllin Ⅰ,Ⅱ, Ⅵ,Ⅶ and Hyperoside in Chonglou Kegan Capsule[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(15): 58-61.
DOI：

YIN Xing-bin, LIN Long-fei, CAO Sa-li, et al. Simultaneous Determination of Polyphyllin Ⅰ,Ⅱ, Ⅵ,Ⅶ and Hyperoside in Chonglou Kegan Capsule[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(15): 58-61. DOI： 10.11653/syfj2013150058.

摘要

目的: 建立重楼皂苷Ⅰ

Ⅱ

Ⅵ

Ⅶ及金丝桃苷的HPLC含量测定方法

并使用本方法测定3批重楼克感胶囊中5种化合物的含量。方法: Dikma C18(2)色谱柱(4.6 mm×250 mm

5 μm)

乙腈-0.1%磷酸 (42:58) 为流动相

检测波长203

360 nm

流速1.0 mL·min-1

柱温30℃。结果: 重楼皂苷Ⅰ

Ⅱ

Ⅵ

Ⅶ及金丝桃苷在0.1~10 μg线性关系均良好

加样回收率为98.91%~102.23%。结论: 建立了重楼皂苷Ⅰ

Ⅱ

Ⅵ

Ⅶ及金丝桃苷的HPLC测定方法

操作方便

结果准确

可用于重楼克感胶囊中5种化合物的含量测定。

Abstract

Objective: A rapid and specific RP-HPLC-UV method was developed for the analysis of polyphyllin Ⅰ

Ⅱ

Ⅵ

Ⅶ and hyperoside. The method was applied to study the content determination of Chonglou Kegan capsule containing polyphyllin Ⅰ

Ⅱ

Ⅵ

Ⅶ and hyperoside. Method: The analysis was carried out on a Diamonsil C18 (2) reversed-phase column (4.6 mm×250 mm

5 μm) by isocratic elution with acetonitrile and 0.1% phosphoric acid (42:58). The flow rate was 1.0 mL·min-1 and the detection wavelength was set at 203

360 nm. Result: Linear responses were obtained for polyphyllin Ⅰ

Ⅱ

Ⅵ

Ⅶ and hyperoside ranging from 0.1 to 10 μg. The extraction recovery ranged from 98.91%-102.12%. Stability studies showed that polyphyllin Ⅰ

Ⅱ

Ⅵ

Ⅶ and hyperoside were stable in preparation and analytical process. Conclusion: The results indicated that the validated method was successfully used to determine the concentration of polyphyllin Ⅰ

Ⅱ

Ⅵ

Ⅶ and hyperoside.

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