Objective: To investigate effects of Andrographis Herba compatibility with Artemisiae Scopariae Herba on content change of andrographolide and dehydroandrographolide. Method: RP-HPLC was performed on an Innersil ODS-3 column (4.6 mm×250 mm

5.0 μm) with mobile phase of acetonitrile-water in gradient elution

flow rate was 1.2 mL·min-1

detection wavelengths were set at 225 nm for andrographolide in 0-10 min and 254 nm for dehydroandrographolide in 10-20 min

column temperature was 35℃. Result: The linear range of andrographolide and dehydroandrographolide were 19.66-314.6(r=0.999 5)

4.3-69.12 g·L-1(r=0.999 5)

respectively.Average recoveries of these two components were 99.7% and 99.9%

respectively with RSD were less than 2.3%. Conclusion: The contents of andrographolide and dehydroandrographolide from Andrographis Herba increased significantly after compatibility with Artemisiae Scopariae Herba.