Determination and Antioxidant Activity Investigation of Polysaccharides from

DU Qing-hua; HUANG Yuan-he; PAN Qiao-dan; WANG Liu-nian; TANG Hai-yan; LIN Qing-yun

doi:10.11653/syfj2013150067

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Determination and Antioxidant Activity Investigation of Polysaccharides from

更新时间：2024-01-04

- Determination and Antioxidant Activity Investigation of Polysaccharides from
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 19, Issue 15, Pages: 67-69(2013)
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- DOI：10.11653/syfj2013150067
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- Published：2013
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DU Qing-hua, HUANG Yuan-he, PAN Qiao-dan, et al. Determination and Antioxidant Activity Investigation of Polysaccharides from [J]. Chinese journal of experimental traditional medical formulae, 2013, 19(15): 67-69.
DOI：

DU Qing-hua, HUANG Yuan-he, PAN Qiao-dan, et al. Determination and Antioxidant Activity Investigation of Polysaccharides from [J]. Chinese journal of experimental traditional medical formulae, 2013, 19(15): 67-69. DOI： 10.11653/syfj2013150067.

摘要

目的: 测定翼齿六棱菊多糖的含量并探讨其体外抗氧化活性。方法: 运用微波-超声波协同提取翼齿六棱菊多糖

采用苯酚-硫酸法测定多糖含量

通过FRAP法、水杨酸法、ABTS法和邻苯三酚法检测翼齿六棱菊多糖的抗氧化能力。结果: 翼齿六棱菊多糖提取量200 mg·g-1;与对照品抗坏血酸和茶多酚相比

翼齿六棱菊多糖的Fe3+还原抗氧化能力(829.75 μmol·g-1)弱于抗坏血酸(1 536.5 μmol·g-1)

强于茶多酚(739.75 μmol·g-1);清除ABTS+自由基能力(IC50 0.197 g·L-1)及清除超氧阴离子自由基能力(IC50 0.289 g·L-1)比抗坏血酸弱(IC50分别为0.032

0.259 g·L-1)

但比茶多酚强(IC50分别为0.233

0.335 g·L-1);清除OH·自由基能力(IC50 0.245 g·L-1)弱于抗坏血酸(IC50 0.199 g·L-1)和茶多酚(IC50 0.233 g·L-1)。结论: 采用苯酚-硫酸比色法测定翼齿六棱菊多糖的含量具有简便、快速、准确、灵敏度好等优点

翼齿六棱菊多糖具有较强的抗氧化能力。

Abstract

Objective: To determine the content of polysaccharides in Laggera pterodonta and investigate its in vitro antioxidative activity. Method: Polysaccharides in L. pterodonta was extracted by ultrasonic-microwave synergistic method

the content of polysaccharides was determined by phenol-sulfuric acid colorimetry

its antioxidant activity was measured by FRAP assay

salicylic acid assay

ABTS assay and adjacent benzene pyrogallol assay. Result: The content of polysaccharides in L. pterodonta was 200 mg·g-1.Compared with the positive control of ascorbic acid and tea polyphenols

reducing Fe3+ antioxidant capacity of polysaccharides in L. Pterodonta (829.75 μmol·g-1) was weaker than ascorbic acid (1 536.5 μmol·g-1)

but higher than tea polyphenols (739.75 μmol·g-1).Scavenging ABTS+radical capacity (IC50 0.197 g·L-1) and superoxide anion radical capacity (IC50 0.289 g·L-1) was lower than ascorbic acid (IC50 were 0.032

0.259 g·L-1

respectively)

but higher than tea polyphenols (IC50 were 0.233

0.335 g·L-1

respectively).Scavenging OH·(IC50 0.245 g·L-1)was lower than ascorbic acid (IC50 0.199 g·L-1) and tea polyphenols (IC50 0.233 g·L-1). Conclusion: Phenol-sulfuric acid colorimetry to determine the content of polysaccharides from L. pterodonta was simple

rapid and accurate with good sensitivity

antioxidant activity of polysaccharides from L. pterodonta was strong.

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