Objective: To establish a HPLC method for the determination of α-linolenic acid

linoleic acid and oleic acid in Oviductus Ranae. Method: Alltima C18(4.6 mm× 250 mm

5 μm) column was used with mobile phase of acetonitrile-0.1% phosphoric acid (80:20);the flow rate was 1 mL·min-1;the column temperature was kept at 30℃;the detection wavelength was set at 203 nm. Result: The linear ranges of α-linolenic acid

linoleic acid and oleic acid were 4.755-152.16 mg·L-1 (r=1)

15.362-491.59 mg·L-1 (r=0.999 8) and 47.275-1512.8 mg·L-1 (r=0.999 9) respectively.The average recoveries were 95.8% with RSD 1.79% for α-linolenic acid

98.6% with RSD 1.32% for linoleic acid

99.3% with RSD 1.59% for oleic acid. Conclusion: The method is simple

accurate

convenient

specific and repeatable

which can be used to control the quality of α-linolenic acid

linoleic acid and oleic acid in Oviductus Ranae.