Identification of Lycopodii Herba by UPLC Fingerprint

YANG Ye; WANG Xiang-pei; SUN Qing-wen; XU Feng

doi:10.11653/syfj2013150117

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Identification of Lycopodii Herba by UPLC Fingerprint

更新时间：2024-01-04

- Identification of Lycopodii Herba by UPLC Fingerprint
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 19, Issue 15, Pages: 117-120(2013)
- 作者机构：
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- DOI：10.11653/syfj2013150117
  CLC：
- Published：2013
- 稿件说明：
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YANG Ye, WANG Xiang-pei, SUN Qing-wen, et al. Identification of Lycopodii Herba by UPLC Fingerprint[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(15): 117-120.
DOI：

YANG Ye, WANG Xiang-pei, SUN Qing-wen, et al. Identification of Lycopodii Herba by UPLC Fingerprint[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(15): 117-120. DOI： 10.11653/syfj2013150117.

摘要

目的: 建立伸筋草药材的超高效液相特征性指纹图谱鉴别方法

为有效控制和科学评价伸筋草药材质量提供依据。方法: 采用Agilent ZORBAX RRHD Eclipse Plus C18(2.1 mm×100 mm

1.8 μm)色谱柱

流动相甲醇-乙腈(1:1)-0.05%磷酸水

梯度洗脱

检测波长256 nm

流速0.1 mL·min-1

测定10批伸筋草药材及混淆品的指纹图谱

并作相似度比较分析。结果: 建立了伸筋草药材UPLC特征指纹图谱共有模式

标定了19个共有峰

10批伸筋草药材的相似度为0.923~0.985

而与混淆品的指纹图谱比较

结果具有明显差异。结论: 方法快速、高效

可用于伸筋草药材的质量评价。

Abstract

Objective: To establish a UPLC and develop an UPLC fingerprint for the identification of Lycopodii Herba for controlling the drug quality quickly and accurately. Method: The UPLC fingerprint of ten batches of Lycopodii Herba and adulterants were determined on an Agilent ZORBAX RRHD Eclipse Plus C18 (2.1 mm×100 mm

1.8 μm) column eluted with the mobile phase consisted of methanol-acetonitrile (1:1)-water containing 0.05% phosphoric acid in gradient mode and the detection wavelength was at 256 nm

the flow rate of 0.1 mL·min-1. Result: The method of HPLC fingerprints was established and the 19 mutual peaks were indicated. The similar degrees of the ten batches were between 0.923-0.985.The similarities among the Lycopodii Herba and adulterants from different sources were obtained

there were apparent difference in fingerprint. Conclusion: The method is quick and accurate. The UPLC fingerprint of Lycopodii Herba with high specificity can be used to control the quality of Lycopodii Herba.

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WANG Yuan-min

ZHENG Xia-sheng

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Related Institution

Institute of Chinese Materia Medica，China Academy of Chinese Medical Sciences

State Key Laboratory of Southwestern Traditional Chinese Medicine（TCM） Resources， School of Pharmacy，Chengdu University of TCM

Sichuan Neo-green Pharmaceutical Technology Development Co. Ltd.，Pengzhou

Guangzhou University of Chinese Medicine

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