RP-HPLC with UV Switch Determination of Paeoniflorin and Pepper Alkali in Zhitong Tablets

WU Jian; JI Ling; WANG Li-fang; XIAO Lin-lin; HE Yong; LAI Yan-ting; LI Li

doi:10.11653/syfj2013150140

您当前的位置：

首页 >

文章列表页 >

RP-HPLC with UV Switch Determination of Paeoniflorin and Pepper Alkali in Zhitong Tablets

更新时间：2024-01-04

- RP-HPLC with UV Switch Determination of Paeoniflorin and Pepper Alkali in Zhitong Tablets
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 19, Issue 15, Pages: 140-142(2013)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.11653/syfj2013150140
  CLC：
- Published：2013
- 稿件说明：
移动端阅览
WU Jian, JI Ling, WANG Li-fang, et al. RP-HPLC with UV Switch Determination of Paeoniflorin and Pepper Alkali in Zhitong Tablets[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(15): 140-142.
DOI：

WU Jian, JI Ling, WANG Li-fang, et al. RP-HPLC with UV Switch Determination of Paeoniflorin and Pepper Alkali in Zhitong Tablets[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(15): 140-142. DOI： 10.11653/syfj2013150140.

摘要

目的: 建立同时测定止痛片中芍药苷和胡椒碱含量的方法。方法: 采用 Phenomsil ODS (4.6 mm×250 mm

5 μm)色谱柱

以乙腈-0.1%磷酸为流动相

梯度洗脱

流速1.0 mL·min-1

检测波长230 nm (0~15 min

芍药苷)

343 nm(15~30 min

胡椒碱)

柱温30℃。结果: 芍药苷在 4.82~48.24 mg·L-1 (r=0.999 8)呈良好的线性关系

平均回收率为100.59%

RSD 1.43% (n=6)

胡椒碱在2.51~25.12 mg·L-1 (r=0.999 7)呈良好的线性关系

平均回收率为99.84%

RSD 1.45% (n=6)。结论: 方法简单

灵敏度高

可用于止痛片中芍药苷和胡椒碱的含量测定。

Abstract

Objective: To establish a simultaneous determination method for paeoniflorin and pepper alkali in Zhitong Tablets. Method: The determination was performed on a Phenomsil ODS column with acetonitrile-0.1% phosphoric acid as the mobile phase. The detection wavelength was set at 230 nm (0-15 min

paeoniflorin) and 343 nm (15-30 min

pepper alkali). The flow rate was 1.0 mL·min-1. The column temperature was maintained at 30℃. Result: paeoniflorin presented a good linearity in the concentration range of 4.82-48.24 mg·L-1 (r=0.999 8). The average recovery rate was 100.59% with RSD 1.43% (n=6). Pepper alkali presented a good linearity in the concentration range of 2.51-25.12 mg·L-1 (r=0.999 7). The average recovery rate was 99.84% with RSD 1.45% (n=6). Conclusion: The method is simple

accurate and reproducible

it can be used as the basis for quality control of Zhitong Tablets.

关键词

Keywords

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Studies on Quality Standard for Danqi Quyu Zhitong Granules

Simultaneous Determination of Paeoniflorin, Salvianolic Acid B and Berberine in Fuyankang Pills by HPLC

HPLC Analysis of the Composition of Albiflorin, Paeoniflorin, 2,3,5,4'-Tetrahydro Xystilbene-2--Glucoside and Penta--Galloyl--Glucoside in Kundan Capsule

Optimization of Smashing Tissue Extraction Process of Total Paeony Glycoside by Orthogonal Test

Studies on Quality Control of Xuanduan Tongluo Capsule

Related Author

WANG Zhao-yu

BAI Xiu-yun

BAI Hai-yu

WANG Wei-ming

HUANG Ming-qing

XIE You-liang

WANG De-hang

JIANG Dong-xu

Related Institution

Shanxi University of Traditional Chinese Medicine

Hangzhou Normal University

Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences

Heilongjiang Academy of Traditional Chinese Medicine

The Affiliated Cancer Hospital of Zhengzhou University/Henan Cancer Hospital

AI问答

Postal code：100700
Tel：010-84076882 Email：syfjx_2010@188.com
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备11006657号-10 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰