Identification of  and Common Adulterants by DNA Fingerprint

CHI Ying; ZHOU Lin; ZHANG Li-hua; LI Ming-cheng; WANG Bing-mei

doi:10.11653/syfj2013150143

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Identification of and Common Adulterants by DNA Fingerprint

更新时间：2024-01-04

- Identification of and Common Adulterants by DNA Fingerprint
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 19, Issue 15, Pages: 143-147(2013)
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- DOI：10.11653/syfj2013150143
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- Published：2013
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CHI Ying, ZHOU Lin, ZHANG Li-hua, et al. Identification of and Common Adulterants by DNA Fingerprint[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(15): 143-147.
DOI：

CHI Ying, ZHOU Lin, ZHANG Li-hua, et al. Identification of and Common Adulterants by DNA Fingerprint[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(15): 143-147. DOI： 10.11653/syfj2013150143.

摘要

目的: 建立柴胡及其伪品DNA指纹鉴别方法。方法: 采用改良的CTAB法提取柴胡及常见伪品的干根基因组DNA

设计鉴别正品柴胡及其伪品的特异性引物

利用PCR扩增技术对正品柴胡及其伪品进行DNA指纹鉴定。结果: 改良的CTAB法提取出大小为23 kb的基因组DNA条带

纯度在(1.71±0.11)

且经特异引物PCR扩增

只有正品柴胡能扩增出173 bp大小的片段

而伪品未能扩增出目的片段。结论: 柴胡DNA具有特异性指纹特征

可作为市售柴胡的真伪鉴定依据。

Abstract

Objective: To establish DNA fingerprint method for the identification of Bupleurum chinese and adulterants. Method: The improved CTAB method was used to extract genomic DNA from B. chinese and common adulterants

designning the specific primer that identifies the B. chinese and common adulterants

and using DNA fingerprint Technology to identify the B. chinese and common adulterants by PCR. Result: The 23 kb DNA was separated from the B. chinese and common adulterantsthe by the improved CTAB

which purity was (1.71±0.11)

the diagnostic results of PCR with specific primer showed that a 173 bp fragment was only amplified from the B. chinese. Conclusion: B. chinese DNA fingerprint has specific characteristics

it can be used for the crude drugs in the current market.

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Related Author

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Related Institution

School of Pharmacy，Lanzhou University

Key Laboratory of Quality Control of Chinese Medicinal Materials and Decoction Pieces， National Medical Products Administration，Gansu Institute for Drug Control

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