Objective: This article assessed the genetic relationship and genetic diversity in Erodium stephanianum. Method: Twenty-three germplasmic resources of E. Stephanianum. were analyzed by inter simple sequence repeat(ISSR) molecular markers. Genetic similarities were calculated by using NTSYS software and the dendrogram was constructed by using UPGMA method. Result: Fourteen ISSR primers were selected from 100 ISSR primers

and 185 DNA fragments were amplified from 23 samples.Of which

142 fragment were polymorphic(percentage of polymorphic bands was 76.8%). The genetic similarity among all accessions ranged from 0.458 to 0.916.Clustering analysis showed that the 23 samples of Fritillaria could be distinctively classified into 6 groups. Conclusion: Results from the cluster analysis were correlated significantly with the morphological characteristics and geographical location of 23 samples. The data indicate that ISSR technique is useful to determine genetic diversity and genetic relationship among E. stephanianum. provenances

providing a scientific basis for genetic breeding

differentiation and new cultivar selection.