ZHAO Jing-yi, DU Shou-ying, LU Yang, et al. Tissue Distribution of Borneol in Mice after Oral Administration[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(15): 159-161.
DOI:
ZHAO Jing-yi, DU Shou-ying, LU Yang, et al. Tissue Distribution of Borneol in Mice after Oral Administration[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(15): 159-161. DOI: 10.11653/syfj2013150159.
Tissue Distribution of Borneol in Mice after Oral Administration
Objective: To develop a GC-FID method for determination of borneol concentration in mice tissues and investigate its tissues distribution after oral administration. Method: Wholebrain
heart
liver
spleen
lungs and kidneys were quickly isolated by executing mice at 1
3
5
10
20
30
60
90
120 min after oral administration of borneol with a dosage of 30.0 mg·kg-1.These tissues were extracted with ethyl acetate and detected by GC-FID
octadecane was used as an internal standard. Result: Calibration curve was good linear
extraction recoveries
RSD of within-day and between-day with low
medium and high concentration were satisfied for analysis requirements of biological samples.Borneol distributed in all major organs after administration with the order of liver>kidney>heart>brain>spleen>lung
Tmax in liver
lung and spleen was earlier than in heart
brain and kidney. Conclusion: Established GC-FID could be applied to determination of borneol in tissues.There were differences of borneol distribution in each tissues
it mainly distributed in abundant blood-supply tissues such as liver
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Related Author
SUN Jing
QI Yunpeng
LI Siyuan
HU Yingbo
ZHONG Linying
CHEN Jianbo
DONG Ling
ZHANG Jianwei
Related Institution
School of Life Sciences,Beijing University of Chinese Medicine
Third Grade Laboratory of Traditional Chinese Medicine(TCM) Preparations,National Administration of TCM,Shuguang Hospital Affiliated to Shanghai University of TCM
Affiliated Hospital of Nanjing University of Chinese Medicine