Objective: To investigate the anti-tumor activity in vivo of extract from compound Fomes fomentarius (ECF)

and reveal the anti-tumor mechanism of ECF. Method: S180-bearing and Lewis tumor-bearing mice models were established. Mice were divided into five groups

model group

cyclophosphamide (CTX) group

high-(0.15 g·kg-1)

middle-(0.1 g·kg-1)and low-dose (0.05 g·kg-1) of ECF.The S180-bearing and Lewis tumor-bearing mice in every group received corresponding drugs for 10 and 15 days respectively

then the effects of ECF on tumor growth

thymus and spleen indexs

tumor necrosis factor (TNF-α)and macrophage colony-stimulating factor (M-CSF) levels were determined. Result: The inhibition rate of the three groups(low-

middle-

and high-dose group) of ECF in S180-bearing mice was 11.8%

47.1% and 58.8% respectively

and in Lewis tumor-bearing mice

the inhibition rate was 7.1%

57.1% and 28.6% respectively. Compared with model group

high-dose group of ECF could inhibit tumor growth and reduce M-CSF levels of S180-bearing mice (P<0.05)

middle-dose group could inhibit tumor growth and reduce M-CSF levels of Lewis tumor-bearing mice (P<0.05)

high-dose group could increase thymus indexes

high-

middle-and low-dose groups of ECF could elevate TNF-α levels (P<0.05

P<0.01). Conclusion: ECF could exhibit effect on the growth of transplanted S180 and Lewis tumor

probably via increasing indexes of immune organ and the regulation of TNF-α and M-CSF expression.