Regulation of Puerarin and Oleanolic Acid on Antioxidant Activity and Suppressing Inflammatory Reaction in Rats with Type 2 Diabetes Mellitus

WANG Hui-min; TIAN Wei; LA Xiao-jin; GAO Xiu-juan; HAN Ting; WANG Ya; TIAN Zhen-ya; Hegyi Gabriella; Chen Zhen; LI Ji-an

doi:10.11653/syfj2013150174

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Regulation of Puerarin and Oleanolic Acid on Antioxidant Activity and Suppressing Inflammatory Reaction in Rats with Type 2 Diabetes Mellitus

更新时间：2024-01-04

- Regulation of Puerarin and Oleanolic Acid on Antioxidant Activity and Suppressing Inflammatory Reaction in Rats with Type 2 Diabetes Mellitus
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 19, Issue 15, Pages: 174-177(2013)
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- DOI：10.11653/syfj2013150174
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- Published：2013
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WANG Hui-min, TIAN Wei, LA Xiao-jin, et al. Regulation of Puerarin and Oleanolic Acid on Antioxidant Activity and Suppressing Inflammatory Reaction in Rats with Type 2 Diabetes Mellitus[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(15): 174-177.
DOI：

WANG Hui-min, TIAN Wei, LA Xiao-jin, et al. Regulation of Puerarin and Oleanolic Acid on Antioxidant Activity and Suppressing Inflammatory Reaction in Rats with Type 2 Diabetes Mellitus[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(15): 174-177. DOI： 10.11653/syfj2013150174.

摘要

目的: 观察葛根素、齐墩果酸及其配伍对2型糖尿病大鼠血糖、血脂、血清超氧化物歧化酶(SOD)、丙二醛(MDA)、糖基化终末产物(AGEs)、超敏C反应蛋白(hs-CRP)及胰腺病理组织学的影响。方法: 以高糖、高脂饮食联合链脲佐菌素(30 mg·kg-1)ip法建立2型糖尿病大鼠模型

随机分为模型(M)组、二甲双胍(D)组(100 mg·kg-1·d-1)、葛根素(P)组(30 mg·kg-1·d-1)、齐墩果酸(OA)组(10 mg·kg-1·d-1)、葛根素+齐墩果酸(POA)组(40 mg·kg-1·d-1)

同期设正常对照组(K)。各治疗组分别ig 给予相应药物治疗。治疗前及治疗2

6周分别测定各组大鼠空腹血糖(FBG)及餐后2 h血糖(P2hPG)

并于给药6周后

测定大鼠总胆固醇(CHOL)、甘油三酯(TG)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)

hs-CRP

血清SOD

MDA

AGEs水平

光镜下观察胰腺病理组织学变化。结果: M组大鼠FBG

P2hPG

CHOL

LDL-C

hs-CRP

MDA

AGEs较K组升高

HDL-C和血清SOD水平明显下降

有统计学差异

对胰腺胰岛细胞有一定损伤。各治疗组大鼠FBG

P2hPG

CHOL

LDL-C

hs-CRP

MDA

AGEs较M组降低

HDL-C和血清SOD水平增加

对胰腺胰岛细胞损伤有不同程度改善。POA组LDL-C水平较P组、OA组降低明显(P<0.05)

对胰腺胰岛细胞损伤明显改善。结论: 葛根素、齐墩果酸及其配伍可改善实验性2型糖尿病大鼠的糖、脂代谢

其作用机制可能与抗氧化、抑制炎症反应有关。其中葛根素配伍齐墩果酸在降低LDL-C水平、改善胰岛细胞结构方面优于单纯葛根素、齐墩果酸。

Abstract

Objective: To observe effects of puerarin and oleanolic acid on blood glucose

blood lipid

superoxide dismutase (SOD)

malondialdehyde(MDA)

advanced glycation end products (AGEs)

serum hypersensitive C-reactive protein (hs-CRP) and pancreatic histopathology in rats with type 2 diabetes mellitus(T2DM)

and to approach their pharmacological mechanism. Method: T2DM rats induced by high calories diet and intraperitoneal injection of streptozotacin(STZ) were randomly divided into five groups:diabetes mellitus (M)group

melbine(D) group

puerarin(P) group

oleanolic acid group(OA) and puerarin with oleanolic acid(POA)group. Fasting blood glucose (FBG) and 2-hour postprandial blood glucose (P2hPG) of rats in each group were measured before treatment and after 2

6 weeks of administration. After 6 weeks

blood simples were collected for measurement on blood glucose

as well as following indexes

cholesterol (CHOL)

triglyceride (TG)

HDL-cholesterol (HDL-C)

LDL-cholesterol (LDL-C)

hs-CRP

SOD

MDA and AGEs. Pathological changes were observed under light microscope by HE staining. Result: All indexes were inhibited to certain degrees in every treatment group except HDL-C and SOD. The level of LDL-C in POA group decreased more significantly than that in the P group and OA group(P<0.05)

meanwhile

the injury pancreas tissue was improved significantly. Conclusion: The puerarin and oleanolic acid could markedly improve glucose and lipid metabolism in experimental type 2 diabetic rats. It may prove that puerarin with oleanolic acid can relieve the degree of oxidative stress reaction through antioxidant activity and suppressing inflammatory reaction. In terms of decreased LDL-C and improvement of the structure of islet cells

the effects in POA group are more stronger than those in P and OA group.

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