LIU Xiao-meng, LI Feng, MA Jie, et al. Effect of Sini San Containing Serum on GABA-Cl Current of Cortical Neurons in Rats[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(15): 202-205.
DOI:
LIU Xiao-meng, LI Feng, MA Jie, et al. Effect of Sini San Containing Serum on GABA-Cl Current of Cortical Neurons in Rats[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(15): 202-205. DOI: 10.11653/syfj2013150202.
Effect of Sini San Containing Serum on GABA-Cl Current of Cortical Neurons in Rats
Objective: To investigate the mechanism underlying the insomnia-treating effect by Sini San. Method: SD rats were ig given 21.7 g·kg-1·d-1Sini San for 7 d. Serum from the abdominal aorta was collected and added into rats cortical neurons at the final concentration of 10%
cultured at 37℃ for 3 h.Whole-cell recording technique was used to observe the γ-aminobutyric acid A (GABAA) receptor activated Cl- flux(IGABA)of rats cortical neurons cultured with the serum from Sini San-treated rats. Result: A series concentration of γ-aminobutyric acid(GABA)from 0.3-300 μmol·L-1was given
the cortical neurons treated with Sini San-containing serum for 3 h resulted in left shift of GABA concentration-response curve and half maximal effective concentration(EC50)was decreased(P=0.05).Also the peak amplitude (P<0.05)
τ1(P<0.05) and τ2(P<0.05) of IGABA significantly increased. Conclusion: Sini San affects the IGABA of cortical neurons and this may be contributed to the insomnia-treating effect of Sini San.
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