Objective: To investi gate the influence of ultra-filtration extract from the mixture of Angelica sinensis and Hedysarum polybotrys(UFE-AH) on myocardial cell apoptosis induced by adriamycin(ADR) of neonatal rats. Method: Myocardial cells of neonatal rats born 1 to 2 days were primarily cultured by differential adherent method for 48 h at 37℃

5%CO2 incubator

adjusting the cell concentration to 1×106/mL

then randomly divided into five group:normal control group

adriamycin group

low-does

medium-does

and high-does UFE-AH.to detect MTT method was used to inhibition of cell growth rate

and to detect the level of de-oxyribonucleic acid damage (DNA damage) the single cell gel electrophoresis was used

western blot was used to detect the expression of Bcl-2 and Bax. Result: Compared with the normal group

the inhibition of cell growth rate in adriamycin group increased significantly

head DNA%(HDNA%)

tail DNA%(TDNA%)

tail length(TL)

tail moment(TM)

olive tail moment(OTM) were significantly increased

the protein expressions of Bcl-2 were decreased and the protein expressions of Bax were increased significantly (P<0.01);In comparison of the four other groups with model group

UFE-AH

HDNA%

TDNA%

TL

TM

OTM numerical and the protein expressions of Bax

but increase the protein expressions of Bcl-2 of the myocardial ceels in neonatal rats and the radio of Bcl-2/Bax. Conclusion: can reduce the DNA damage of the myocardial cells induced by adriamycin and increase the radio of Bcl-2/Bax

and has the protective effects on myocardial cell apoptosis induced by adriamycin of neonatal rats.