Protective Effect of Sodium Quercetin-7-mono-sulfonate on HO Induced DNA Oxidative Damage

CHEN Zai-rong; ZHAO Xiao-yan; ZHU Yu-xin; SHEN Qu-shen; DAI Zhong

doi:10.11653/syfj2013150243

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Protective Effect of Sodium Quercetin-7-mono-sulfonate on HO Induced DNA Oxidative Damage

更新时间：2024-01-04

- Protective Effect of Sodium Quercetin-7-mono-sulfonate on HO Induced DNA Oxidative Damage
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 19, Issue 15, Pages: 243-246(2013)
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- DOI：10.11653/syfj2013150243
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- Published：2013
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CHEN Zai-rong, ZHAO Xiao-yan, ZHU Yu-xin, et al. Protective Effect of Sodium Quercetin-7-mono-sulfonate on HO Induced DNA Oxidative Damage[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(15): 243-246.
DOI：

CHEN Zai-rong, ZHAO Xiao-yan, ZHU Yu-xin, et al. Protective Effect of Sodium Quercetin-7-mono-sulfonate on HO Induced DNA Oxidative Damage[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(15): 243-246. DOI： 10.11653/syfj2013150243.

摘要

目的: 探讨槲皮素-7-硫酸酯钠的体外抗氧化活性及对DNA氧化损伤的保护作用。方法: 通过1

1-二苯基-2-三硝基苯肼(DPPH)自由基(·DPPH)、羟自由基(OH·)和超氧阴离子自由基(O2-·)清除实验评价槲皮素-7-硫酸酯钠的体外抗氧化活性;采用紫外扫描法、凝胶电泳技术观察槲皮素-7-硫酸酯钠对H2O2所致DNA氧化损伤的保护作用。结果: 自由基清除实验结果显示

槲皮素-7-硫酸酯钠能有效清除·DPPH、·OH和O2-·自由基

其清除能力较维生素C(VC)及槲皮素都强

且呈浓度依赖性。DNA氧化损伤保护实验结果显示

H2O2可致小牛胸腺DNA出现断裂梯带和浓度下降

槲皮素-7-硫酸酯钠预处理可抑制H2O2所致的小牛胸腺DNA断裂及浓度下降

且有浓度依赖性。结论: 槲皮素-7-硫酸酯钠体外有较强的抗氧化能力

并对H2O2所致的DNA氧化损伤有保护作用。

Abstract

Objective: To provide a theoretical basis for clinical application of sodium quercetin-7-mono-sulfonate (SQMS)

its antioxidant property and DNA oxidative damage protective effect in vitro were studied. Method: The antioxidant activity of SQMS was evaluated by 1

1-diphenyl-2-picryl-hydrazyl (·DPPH)

hydroxyl radical (OH·) and superoxide radical (·O2-) scavenging assays. Gel electrophoresis and spectrophotometry methods were used to study the protective effect of SQMS on H2O2 induced DNA oxidative damage in vitro. Result: SQMS exhibited a concentration-dependent scavenging effect on·DPPH

·OH and O2-·

and its scavenging effects are stronger than vitamin C(VC) and quercetin. By gel electrophoresis and spectrophotometry

the DNA ladder disappeared and the DNA concentration obvious increased by treatment with H2O2.Pretreatment with quercetin or sodium quercetin-7-sulfonate

the DNA ladder was more clear and the concentration of DNA decreased obviously. Conclusion: SQMS has stronger antioxidant activity in vitro

and has protective effect on H2O2induced DNA oxidative damage.

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