Effects of Bushen Tongluo Decoction on OPG/RANKL mRNA Expression of in Osteoporosis Rats

MIN Wen; HUANG Gui-cheng; HUA Yong-qin; HUANG Zheng-quan

doi:10.11653/syfj2013150258

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Effects of Bushen Tongluo Decoction on OPG/RANKL mRNA Expression of in Osteoporosis Rats

更新时间：2024-01-04

- Effects of Bushen Tongluo Decoction on OPG/RANKL mRNA Expression of in Osteoporosis Rats
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 19, Issue 15, Pages: 258-261(2013)
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- DOI：10.11653/syfj2013150258
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- Published：2013
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MIN Wen, HUANG Gui-cheng, HUA Yong-qin, et al. Effects of Bushen Tongluo Decoction on OPG/RANKL mRNA Expression of in Osteoporosis Rats[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(15): 258-261.
DOI：

MIN Wen, HUANG Gui-cheng, HUA Yong-qin, et al. Effects of Bushen Tongluo Decoction on OPG/RANKL mRNA Expression of in Osteoporosis Rats[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(15): 258-261. DOI： 10.11653/syfj2013150258.

摘要

目的: 研究补肾通络方对去卵巢骨质疏松模型大鼠细胞核因子κΒ受体活化因子配体(RANKL)/骨保护素(OPG)基因表达的影响

探讨补肾通络方治疗原发性骨质疏松症的分子机制。方法: 通过卵巢去势的方法造成大鼠骨质疏松模型

造模后随机分为6组:假手术组、模型组、仙灵骨葆组(5.0 g·kg-1)、补肾基础组(5.4 g·kg-1)、通络组(0.9 g·kg-1)、补肾通络组(6.3 g·kg-1)。ig给药

1次/d

共10周。治疗10周后

在无菌条件下取出大鼠L3椎体

剔除软组织及骨膜

用逆转录聚合酶链式反应(RT-PCR)检测骨组织RANKL/OPG基因mRNA的表达。结果: 与模型组相比

补肾通络组、补肾基础组、通络组RANKL mRNA表达明显下降

RANKL/OPG明显下降(P<0.01)

3组间比较

补肾通络组的干预作用明显优于补肾基础组与通络组(P<0.05)。结论: 补肾通络方治疗原发性骨质疏松症的作用机制可能与调控RANKL mRNA表达

改善RANKL/OPG

从而抑制破骨细胞活性

降低骨吸收有关。

Abstract

Objective: To investigate the effects of Bushen Tongluo decoction (BTD) on mRNA expression of receptor activator of NF-κΒ lingand (RANKL)/osteoprotegerin system (OPG) in the osteoporosis rats by bilateral ovariotomy and explore the molecular mechanism of treating osteoporosis with BTD. Method: Rat model of the osteoporosis was established by bilateral ovariotomy. The rats were divided into six groups randomly:the sham operated group

model group

Xianling Gubao group(5.0 g·kg-1)

Bushen decoction group(5.4 g·kg-1)

Tongluo decoction group(0.9 g·kg-1)

BTD group(6.3 g·kg-1). After ten weeks treatment. The mRNA expression of OPG and RANKL in L3 bone tissues was analyzed by RT-PCR. Result: BTD group

Bushen decoction group and Tongluo decoction group could reduce RANKL mRNA expression

decrease the ratio of RANKL/OPG compared with model group

and there was significant difference(P<0.01).The effect of BTD was superior to Bushen decoction and Tongluo decoction(P<0.05). Conclusion: The effect and related mechanism of BTD in the treatment of primary osteoporosis may be related to regulation of RANKL mRNA and improve RANKL/OPG ratio

thereby inhibiting osteoclast activity and reducing bone resorption.

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Related Institution

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Beijing University of Chinese Medicine

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